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• Esposito, R
• Della Ventura, B
• De Nicola, S
• Altucci, C
• Velotta, R
• Mita, D
• Lepore, M
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• Esposito, R
• Della Ventura, B
• De Nicola, S
• Altucci, C
• Velotta, R
• Mita, D
• Lepore, M
• [+] on PubMed
• Esposito, R
• Della Ventura, B
• De Nicola, S
• Altucci, C
• Velotta, R
• Mita, D
• Lepore, M

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Sensors 2011, 11(4), 3483-3497; doi:10.3390/s110403483

Article

Glucose Sensing by Time-Resolved Fluorescence of Sol-Gel Immobilized Glucose Oxidase

Rosario Esposito ^1,* , Bartolomeo Della Ventura ², Sergio De Nicola ³, Carlo Altucci ¹, Raffaele Velotta ¹, Damiano Gustavo Mita ^2,4 and Maria Lepore ²

¹ CNISM-Dipartimento Scienze Fisiche, Università di Napoli Federico II, Napoli 80126, Italy ² Dipartimento di Medicina Sperimentale, Seconda Università di Napoli, Napoli 80138, Italy ³ Istituto Nazionale di Ottica, CNR, compr. A. Olivetti, Via Campi Flegrei 34, Pozzuoli 80078, Italy ⁴ Institute of Genetics and Biophysics, CNR, Naples 80131, Italy

* Author to whom correspondence should be addressed.

Received: 11 February 2011; in revised form: 14 March 2011 / Accepted: 16 March 2011 / Published: 24 March 2011

(This article belongs to the Section Biosensors)

Download PDF Full-Text [1640 KB, uploaded 24 March 2011 17:25 CET]

Abstract: A monolithic silica gel matrix with entrapped glucose oxidase (GOD) was constructed as a bioactive element in an optical biosensor for glucose determination. Intrinsic fluorescence of free and immobilised GOD was investigated in the visible range in presence of different glucose concentrations by time-resolved spectroscopy with time-correlated single-photon counting detector. A three-exponential model was used for analysing the fluorescence transients. Fractional intensities and mean lifetime were shown to be sensitive to the enzymatic reaction and were used for obtaining calibration curve for glucose concentration determination. The sensing system proposed achieved high resolution (up to 0:17 mM) glucose determination with a detection range from 0:4 mM to 5 mM.

Keywords: glucose oxidase; biosensors; glucose determination; time-resolved fluorescence; lifetime

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