Sensors 2011, 11(12), 11335-11342; doi:10.3390/s111211335
Article

Time-Resolved Fluorescence Immunoassay for C-Reactive Protein Using Colloidal Semiconducting Nanoparticles

1,* email, 1,2email, 1,3email, 1email and 4email
Received: 24 August 2011; in revised form: 18 October 2011 / Accepted: 14 November 2011 / Published: 28 November 2011
(This article belongs to the Special Issue Sensing with Quantum Dots)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Besides the typical short-lived fluorescence with decay times in the nanosecond range, colloidal II/VI semiconductor nanoparticles dispersed in buffer also possess a long-lived fluorescence component with decay times in the microsecond range. Here, the signal intensity of the long-lived luminescence at microsecond range is shown to increase 1,000-fold for CdTe nanoparticles in PBS buffer. This long-lived fluorescence can be conveniently employed for time-gated fluorescence detection, which allows for improved signal-to-noise ratio and thus the use of low concentrations of nanoparticles. The detection principle is demonstrated with a time-resolved fluorescence immunoassay for the detection of C-reactive protein (CRP) using CdSe-ZnS nanoparticles and green light excitation.
Keywords: colloidal nanoparticles; quantum dots; time-gated fluorescence; sensing; bioaffinity assays
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MDPI and ACS Style

Härmä, H.; Toivonen, J.; Soini, J.T.; Hänninen, P.; Parak, W.J. Time-Resolved Fluorescence Immunoassay for C-Reactive Protein Using Colloidal Semiconducting Nanoparticles. Sensors 2011, 11, 11335-11342.

AMA Style

Härmä H, Toivonen J, Soini JT, Hänninen P, Parak WJ. Time-Resolved Fluorescence Immunoassay for C-Reactive Protein Using Colloidal Semiconducting Nanoparticles. Sensors. 2011; 11(12):11335-11342.

Chicago/Turabian Style

Härmä, Harri; Toivonen, Juha; Soini, Juhani T.; Hänninen, Pekka; Parak, Wolfgang J. 2011. "Time-Resolved Fluorescence Immunoassay for C-Reactive Protein Using Colloidal Semiconducting Nanoparticles." Sensors 11, no. 12: 11335-11342.

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