Next Article in Journal
A Hierarchical Communication Architecture for Oceanic Surveillance Applications
Next Article in Special Issue
CdTe and CdSe Quantum Dots Cytotoxicity: A Comparative Study on Microorganisms
Previous Article in Journal
On the Biomimetic Design of Agile-Robot Legs
Previous Article in Special Issue
Overview of Stabilizing Ligands for Biocompatible Quantum Dot Nanocrystals
Article Menu

Export Article

Open AccessArticle
Sensors 2011, 11(12), 11335-11342; doi:10.3390/s111211335

Time-Resolved Fluorescence Immunoassay for C-Reactive Protein Using Colloidal Semiconducting Nanoparticles

1
Laboratory of Biophysics, University of Turku, 20520 Turku, Finland
2
Department of Physics, Tampere University of Technology, 33101 Tampere, Finland
3
Turku University of Applied Sciences, 20520 Turku, Finland
4
Fachbereich Physik and Wissenschaftliches Zentrum für Materialwissenschaften, Philipps Universität Marburg., 35037 Marburg, Germany
*
Author to whom correspondence should be addressed.
Received: 24 August 2011 / Revised: 18 October 2011 / Accepted: 14 November 2011 / Published: 28 November 2011
(This article belongs to the Special Issue Sensing with Quantum Dots)
View Full-Text   |   Download PDF [207 KB, uploaded 21 June 2014]   |  

Abstract

Besides the typical short-lived fluorescence with decay times in the nanosecond range, colloidal II/VI semiconductor nanoparticles dispersed in buffer also possess a long-lived fluorescence component with decay times in the microsecond range. Here, the signal intensity of the long-lived luminescence at microsecond range is shown to increase 1,000-fold for CdTe nanoparticles in PBS buffer. This long-lived fluorescence can be conveniently employed for time-gated fluorescence detection, which allows for improved signal-to-noise ratio and thus the use of low concentrations of nanoparticles. The detection principle is demonstrated with a time-resolved fluorescence immunoassay for the detection of C-reactive protein (CRP) using CdSe-ZnS nanoparticles and green light excitation. View Full-Text
Keywords: colloidal nanoparticles; quantum dots; time-gated fluorescence; sensing; bioaffinity assays colloidal nanoparticles; quantum dots; time-gated fluorescence; sensing; bioaffinity assays
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Härmä, H.; Toivonen, J.; Soini, J.T.; Hänninen, P.; Parak, W.J. Time-Resolved Fluorescence Immunoassay for C-Reactive Protein Using Colloidal Semiconducting Nanoparticles. Sensors 2011, 11, 11335-11342.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top