Next Article in Journal
A Hierarchical Communication Architecture for Oceanic Surveillance Applications
Next Article in Special Issue
CdTe and CdSe Quantum Dots Cytotoxicity: A Comparative Study on Microorganisms
Previous Article in Journal
On the Biomimetic Design of Agile-Robot Legs
Previous Article in Special Issue
Overview of Stabilizing Ligands for Biocompatible Quantum Dot Nanocrystals
Sensors 2011, 11(12), 11335-11342; doi:10.3390/s111211335
Article

Time-Resolved Fluorescence Immunoassay for C-Reactive Protein Using Colloidal Semiconducting Nanoparticles

1,* , 1,2
,
1,3
,
1
 and
4
1 Laboratory of Biophysics, University of Turku, 20520 Turku, Finland 2 Department of Physics, Tampere University of Technology, 33101 Tampere, Finland 3 Turku University of Applied Sciences, 20520 Turku, Finland 4 Fachbereich Physik and Wissenschaftliches Zentrum für Materialwissenschaften, Philipps Universität Marburg., 35037 Marburg, Germany
* Author to whom correspondence should be addressed.
Received: 24 August 2011 / Revised: 18 October 2011 / Accepted: 14 November 2011 / Published: 28 November 2011
(This article belongs to the Special Issue Sensing with Quantum Dots)
View Full-Text   |   Download PDF [207 KB, uploaded 21 June 2014]   |   Browse Figures
SciFeed

Abstract

Besides the typical short-lived fluorescence with decay times in the nanosecond range, colloidal II/VI semiconductor nanoparticles dispersed in buffer also possess a long-lived fluorescence component with decay times in the microsecond range. Here, the signal intensity of the long-lived luminescence at microsecond range is shown to increase 1,000-fold for CdTe nanoparticles in PBS buffer. This long-lived fluorescence can be conveniently employed for time-gated fluorescence detection, which allows for improved signal-to-noise ratio and thus the use of low concentrations of nanoparticles. The detection principle is demonstrated with a time-resolved fluorescence immunoassay for the detection of C-reactive protein (CRP) using CdSe-ZnS nanoparticles and green light excitation.
Keywords: colloidal nanoparticles; quantum dots; time-gated fluorescence; sensing; bioaffinity assays colloidal nanoparticles; quantum dots; time-gated fluorescence; sensing; bioaffinity assays
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote |
RIS
MDPI and ACS Style

Härmä, H.; Toivonen, J.; Soini, J.T.; Hänninen, P.; Parak, W.J. Time-Resolved Fluorescence Immunoassay for C-Reactive Protein Using Colloidal Semiconducting Nanoparticles. Sensors 2011, 11, 11335-11342.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert