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Sensors 2011, 11(12), 11335-11342; doi:10.3390/s111211335
Article

Time-Resolved Fluorescence Immunoassay for C-Reactive Protein Using Colloidal Semiconducting Nanoparticles

1,* , 1,2
, 1,3
, 1
 and 4
1 Laboratory of Biophysics, University of Turku, 20520 Turku, Finland 2 Department of Physics, Tampere University of Technology, 33101 Tampere, Finland 3 Turku University of Applied Sciences, 20520 Turku, Finland 4 Fachbereich Physik and Wissenschaftliches Zentrum für Materialwissenschaften, Philipps Universität Marburg., 35037 Marburg, Germany
* Author to whom correspondence should be addressed.
Received: 24 August 2011 / Revised: 18 October 2011 / Accepted: 14 November 2011 / Published: 28 November 2011
(This article belongs to the Special Issue Sensing with Quantum Dots)
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Abstract

Besides the typical short-lived fluorescence with decay times in the nanosecond range, colloidal II/VI semiconductor nanoparticles dispersed in buffer also possess a long-lived fluorescence component with decay times in the microsecond range. Here, the signal intensity of the long-lived luminescence at microsecond range is shown to increase 1,000-fold for CdTe nanoparticles in PBS buffer. This long-lived fluorescence can be conveniently employed for time-gated fluorescence detection, which allows for improved signal-to-noise ratio and thus the use of low concentrations of nanoparticles. The detection principle is demonstrated with a time-resolved fluorescence immunoassay for the detection of C-reactive protein (CRP) using CdSe-ZnS nanoparticles and green light excitation.
Keywords: colloidal nanoparticles; quantum dots; time-gated fluorescence; sensing; bioaffinity assays colloidal nanoparticles; quantum dots; time-gated fluorescence; sensing; bioaffinity assays
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Härmä, H.; Toivonen, J.; Soini, J.T.; Hänninen, P.; Parak, W.J. Time-Resolved Fluorescence Immunoassay for C-Reactive Protein Using Colloidal Semiconducting Nanoparticles. Sensors 2011, 11, 11335-11342.

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