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Sensors 2011, 11(10), 9520-9531; doi:10.3390/s111009520

A Label-Free Electrochemical Immunosensor for Carbofuran Detection Based on a Sol-Gel Entrapped Antibody

School of Agriculture and Food Engineering, Shandong University of Technology, NO.12, Zhangzhou Road, Zibo 255049, Shandong Province, China
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Received: 27 June 2011 / Revised: 1 September 2011 / Accepted: 15 September 2011 / Published: 10 October 2011
(This article belongs to the Section Biosensors)
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Abstract

In this study, an anti-carbofuran monoclonal antibody (Ab) was immobilized on the surface of a glassy carbon electrode (GCE) using silica sol-gel (SiSG) technology. Thus, a sensitive, label-free electrochemical immunosensor for the direct determination of carbofuran was developed. The electrochemical performance of immunoreaction of antigen with the anti-carbofuran monoclonal antibody was investigated by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS), in which phosphate buffer solution containing [Fe(CN)6]3−/4− was used as the base solution for test. Because the complex formed by the immunoreaction hindered the diffusion of [Fe(CN)6]3−/4− on the electrode surface, the redox peak current of the immunosensor in the CV obviously decreased with the increase of the carbofuran concentration. The pH of working solution, the concentration of Ab and the incubation time of carbofuran were studied to ensure the sensitivity and conductivity of the immunosensor. Under the optimal conditions, the linear range of the proposed immunosensor for the determination of carbofuran was from 1 ng/mL to 100 μg/mL and from 50 μg/mL to 200 μg/mL with a detection limit of 0.33 ng/mL (S/N = 3). The proposed immunosensor exhibited good high sensitivity and stability, and it was thus suitable for trace detection of carbofuran pesticide residues. View Full-Text
Keywords: immunosensor; amperometric; label-free; sol-gel; carbofuran immunosensor; amperometric; label-free; sol-gel; carbofuran
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Sun, X.; Du, S.; Wang, X.; Zhao, W.; Li, Q. A Label-Free Electrochemical Immunosensor for Carbofuran Detection Based on a Sol-Gel Entrapped Antibody. Sensors 2011, 11, 9520-9531.

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