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Sensors 2010, 10(6), 5409-5424; doi:10.3390/s100605409

Immunosensor Incorporating Anti-His (C-term) IgG F(ab’) Fragments Attached to Gold Nanorods for Detection of His-Tagged Proteins in Culture Medium

1
Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-747 Olsztyn, Poland
2
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, 02-106 Warsaw, Poland
*
Author to whom correspondence should be addressed.
Received: 1 April 2010 / Revised: 13 May 2010 / Accepted: 20 May 2010 / Published: 1 June 2010
(This article belongs to the Special Issue Bio-devices and Materials)
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Abstract

Immunosensors based on gold electrodes (electrochemical) or gold discs (optical) modified with 1,6-hexanedithiol, gold nanorods and Anti-His (C-term) monoclonal antibody F(ab’) fragment are described. The antigen detected by the sensing platform is a recombinant histidine-tagged silk proteinase inhibitor (rSPI2-His6). Electrochemical impedance spectroscopy (EIS) and surface plasmon resonance (SPR) techniques were used as methods for detection of the antigen. This approach allows to detect the antigen protein in concentration of 10 pg per mL (0.13 pM) of culture medium. The immunosensor shows good reproducibility due to covalent immobilization of F(ab’) fragments to gold nanorods layer
Keywords: His-tag; F(ab’); gold electrodes; nanorods His-tag; F(ab’); gold electrodes; nanorods
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Wąsowicz, M.; Milner, M.; Radecka, D.; Grzelak, K.; Radecka, H. Immunosensor Incorporating Anti-His (C-term) IgG F(ab’) Fragments Attached to Gold Nanorods for Detection of His-Tagged Proteins in Culture Medium. Sensors 2010, 10, 5409-5424.

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