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Quantitative Determination of Fibrinogen of Patients with Coronary Heart Diseases through Piezoelectric Agglutination Sensor
Laboratory, The Clinical Experimental Base of Biosensor and Microarray, The Center of Molecule and Gene Diagnosis, Southwest Hospital, Third Military Medical University, Chongqing 400038, China
The 26th Electronics Research Institute, National Information Industry Department, Chongqing 400060, China
* Authors to whom correspondence should be addressed.
Received: 8 January 2010; in revised form: 8 February 2010 / Accepted: 1 March 2010 / Published: 16 March 2010
Abstract: Fibrinogen can transform fibrin through an agglutination reaction, finally forming fibrin polymer with grid structure. The density and viscosity of the reaction system changes drastically during the course of agglutination. In this research, we apply an independently-developed piezoelectric agglutination sensor to detect the fibrinogen agglutination reaction in patients with coronary heart diseases. The terminal judgment method of determining plasma agglutination reaction through piezoelectric agglutination sensor was established. In addition, the standard curve between plasma agglutination time and fibrinogen concentration was established to determinate fibrinogen content quantitatively. The results indicate the close correlation between the STAGO paramagnetic particle method and the method of piezoelectric agglutination sensor for the detection of Fibrinogen. The correlation coefficient was 0.91 (γ = 0.91). The determination can be completed within 10 minutes. The fibrinogen concentration in the coronary heart disease group was significantly higher than that of the healthy control group (P < 0.05). The results reveal that high fibrinogen concentration is closely correlated to the incurrence, development and prognosis of coronary heart diseases. Compared with other traditional methods, the method of piezoelectric agglutination sensor has some merits such as operation convenience, small size, low cost, quick detecting, good precision and the common reacting agents with paramagnetic particle method.
Keywords: piezoelectric sensor; fibrinogen in plasma; coronary heart diseases; agglutination reaction
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MDPI and ACS Style
Chen, Q.; Hua, X.; Fu, W.; Liu, D.; Chen, M.; Cai, G. Quantitative Determination of Fibrinogen of Patients with Coronary Heart Diseases through Piezoelectric Agglutination Sensor. Sensors 2010, 10, 2107-2118.
Chen Q, Hua X, Fu W, Liu D, Chen M, Cai G. Quantitative Determination of Fibrinogen of Patients with Coronary Heart Diseases through Piezoelectric Agglutination Sensor. Sensors. 2010; 10(3):2107-2118.
Chen, Qinghai; Hua, Xing; Fu, Weiling; Liu, Dongbo; Chen, Ming; Cai, Guoru. 2010. "Quantitative Determination of Fibrinogen of Patients with Coronary Heart Diseases through Piezoelectric Agglutination Sensor." Sensors 10, no. 3: 2107-2118.