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Raman Spectroscopy and Related Techniques in Biomedicine
School of Engineering, The University of Edinburgh, Edinburgh EH9 3JL, UK
* Author to whom correspondence should be addressed.
Received: 27 January 2010; in revised form: 21 February 2010 / Accepted: 1 March 2010 / Published: 9 March 2010
Abstract: In this review we describe label-free optical spectroscopy techniques which are able to non-invasively measure the (bio)chemistry in biological systems. Raman spectroscopy uses visible or near-infrared light to measure a spectrum of vibrational bonds in seconds. Coherent anti-Stokes Raman (CARS) microscopy and stimulated Raman loss (SRL) microscopy are orders of magnitude more efficient than Raman spectroscopy, and are able to acquire high quality chemically-specific images in seconds. We discuss the benefits and limitations of all techniques, with particular emphasis on applications in biomedicine—both in vivo (using fiber endoscopes) and in vitro (in optical microscopes).
Keywords: Raman spectroscopy; CARS microscopy; Raman imaging
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MDPI and ACS Style
Downes, A.; Elfick, A. Raman Spectroscopy and Related Techniques in Biomedicine. Sensors 2010, 10, 1871-1889.
Downes A, Elfick A. Raman Spectroscopy and Related Techniques in Biomedicine. Sensors. 2010; 10(3):1871-1889.
Downes, Andrew; Elfick, Alistair. 2010. "Raman Spectroscopy and Related Techniques in Biomedicine." Sensors 10, no. 3: 1871-1889.