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Sensors 2010, 10(12), 11352-11368; doi:10.3390/s101211352
Review

Sensing-Applications of Surface-Based Single Vesicle Arrays

1,2,3 and 1,2,3,4,*
Received: 23 October 2010; in revised form: 30 November 2010 / Accepted: 7 December 2010 / Published: 13 December 2010
(This article belongs to the Special Issue State-of-the-Art Sensors Technology in Denmark)
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Abstract: A single lipid vesicle can be regarded as an autonomous ultra-miniaturised 3D biomimetic “scaffold” (Ø ≥ 13 nm) ideally suited for reconstitution and interrogation of biochemical processes. The enclosing lipid bilayer membrane of a vesicle can be applied for studying binding (protein/lipid or receptor/ligand interactions) or transmembrane events (membrane permeability or ion channel activation) while the aqueous vesicle lumen can be used for confining few or single macromolecules and probe, e.g., protein folding, catalytic pathways of enzymes or more complex biochemical reactions, such as signal transduction cascades. Immobilisation (arraying) of single vesicles on a solid support is an extremely useful technique that allows detailed characterisation of vesicle preparations using surface sensitive techniques, in particular fluorescence microscopy. Surface-based single vesicle arrays allow a plethora of prototypic sensing applications in a high throughput format with high spatial and high temporal resolution. In this review we present a series of applications of single vesicle arrays for screening/sensing of: membrane curvature dependent protein-lipid interactions, bilayer tension, reactions triggered in the vesicle lumen, the activity of transmembrane protein channels and biological membrane fusion reactions.
Keywords: vesicles; single vesicles; model membrane systems; nanoreactors vesicles; single vesicles; model membrane systems; nanoreactors
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Christensen, S.M.; Stamou, D.G. Sensing-Applications of Surface-Based Single Vesicle Arrays. Sensors 2010, 10, 11352-11368.

AMA Style

Christensen SM, Stamou DG. Sensing-Applications of Surface-Based Single Vesicle Arrays. Sensors. 2010; 10(12):11352-11368.

Chicago/Turabian Style

Christensen, Sune M.; Stamou, Dimitrios G. 2010. "Sensing-Applications of Surface-Based Single Vesicle Arrays." Sensors 10, no. 12: 11352-11368.


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