Next Article in Journal
Reliable Location-Based Services from Radio Navigation Systems
Previous Article in Journal
Determination of Silver(I) by Differential Pulse Voltammetry Using a Glassy Carbon Electrode Modified with Synthesized N-(2-Aminoethyl)-4,4'-Bipyridine
Previous Article in Special Issue
Conducting Polymer 3D Microelectrodes
Sensors 2010, 10(12), 11352-11368; doi:10.3390/s101211352

Sensing-Applications of Surface-Based Single Vesicle Arrays

1,2,3 and 1,2,3,4,*
Received: 23 October 2010 / Revised: 30 November 2010 / Accepted: 7 December 2010 / Published: 13 December 2010
(This article belongs to the Special Issue State-of-the-Art Sensors Technology in Denmark)
View Full-Text   |   Download PDF [1718 KB, uploaded 21 June 2014]   |   Browse Figures


A single lipid vesicle can be regarded as an autonomous ultra-miniaturised 3D biomimetic “scaffold” (Ø ≥ 13 nm) ideally suited for reconstitution and interrogation of biochemical processes. The enclosing lipid bilayer membrane of a vesicle can be applied for studying binding (protein/lipid or receptor/ligand interactions) or transmembrane events (membrane permeability or ion channel activation) while the aqueous vesicle lumen can be used for confining few or single macromolecules and probe, e.g., protein folding, catalytic pathways of enzymes or more complex biochemical reactions, such as signal transduction cascades. Immobilisation (arraying) of single vesicles on a solid support is an extremely useful technique that allows detailed characterisation of vesicle preparations using surface sensitive techniques, in particular fluorescence microscopy. Surface-based single vesicle arrays allow a plethora of prototypic sensing applications in a high throughput format with high spatial and high temporal resolution. In this review we present a series of applications of single vesicle arrays for screening/sensing of: membrane curvature dependent protein-lipid interactions, bilayer tension, reactions triggered in the vesicle lumen, the activity of transmembrane protein channels and biological membrane fusion reactions.
Keywords: vesicles; single vesicles; model membrane systems; nanoreactors vesicles; single vesicles; model membrane systems; nanoreactors
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
MDPI and ACS Style

Christensen, S.M.; Stamou, D.G. Sensing-Applications of Surface-Based Single Vesicle Arrays. Sensors 2010, 10, 11352-11368.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here


Cited By

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert