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Diversity 2017, 9(4), 43; doi:10.3390/d9040043

Staphylococcus aureus Sequences from Osteomyelitic Specimens of a Pathological Bone Collection from Pre-Antibiotic Times

1
Department of Historical Anthropology and Human Ecology, Johann-Friedrich-Blumenbach Institute for Zoology and Anthropology, University of Goettingen, Goettingen 37073, Germany
2
Robert Koch Institute, National Reference Center for staphylococci and enterococci, Wernigerode 38855, Germany
*
Author to whom correspondence should be addressed.
Received: 27 July 2017 / Revised: 15 September 2017 / Accepted: 28 September 2017 / Published: 1 October 2017
(This article belongs to the Special Issue Ancient DNA)
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Abstract

Staphylococcus aureus is a major pathogen causing osteomyelitis, amongst other diseases, and its methicillin-resistant form (MRSA) in particular poses a huge threat to public health. To increase our knowledge of the origin and evolution of S. aureus, genetic studies of historical microorganisms may be beneficial. Thus, the aim of this study was to investigate whether osteomyelitic skeletal material (autopsy specimens collected from the mid 19th century until the 1920s) is suitable for detecting historical S. aureus DNA sequences. We established a PCR-based analysis system targeting two specific genes of S. aureus (nuc and fib). We successfully amplified the historical S. aureus nuc and fib sequences for six and seven pre-antibiotic, osteomyelitic bone specimens, respectively. These results encourage further investigations of historical S. aureus genomes that may increase our understanding of pathogen evolution in relation to anthropogenically introduced antibiotics. View Full-Text
Keywords: Staphylococcus aureus; osteomyelitis; ancient DNA; pathological bone collection; skeletal material; historical pathogens; infectious diseases; PCR; pre-antibiotic era Staphylococcus aureus; osteomyelitis; ancient DNA; pathological bone collection; skeletal material; historical pathogens; infectious diseases; PCR; pre-antibiotic era
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Flux, A.L.; Mazanec, J.; Strommenger, B.; Hummel, S. Staphylococcus aureus Sequences from Osteomyelitic Specimens of a Pathological Bone Collection from Pre-Antibiotic Times. Diversity 2017, 9, 43.

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