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Diversity 2016, 8(2), 8; doi:10.3390/d8020008

Genetic Diversity Assessment of Portuguese Cultivated Vicia faba L. through IRAP Markers

1
Linking Landscape, Environment, Agriculture and Food (LEAF), Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda 1349-017 Lisboa, Portugal
2
Plant Biology/CIBIO-Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto, Campus Agrário de Vairão, 4485—661 Vairão, Portugal
3
Instituto de Agricultura Sostenible, CSIC, Apdo 4084, 14080 Cordoba, Spain
4
Unidade de Investigação de Biotecnologia e Recursos Genéticos, INIAV, Quinta do Marquês, 2784—505 Oeiras, Portugal
*
Author to whom correspondence should be addressed.
Academic Editor: Michael Wink
Received: 18 December 2015 / Revised: 18 March 2016 / Accepted: 21 March 2016 / Published: 25 March 2016
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Abstract

Faba bean have been grown in Portugal for a long time and locally adapted populations are still maintained on farm. The genetic diversity of four Portuguese faba bean populations that are still cultivated in some regions of the country was evaluated using the Inter Retrotransposons Amplified Polymorphism (IRAP) technique. It was shown that molecular markers based on retrotransposons previously identified in other species can be efficiently used in the genetic variability assessment of Vicia faba. The IRAP experiment targeting Athila yielded the most informative banding patterns. Cluster analysis using the neighbor-joining algorithm generated a dendrogram that clearly shows the distribution pattern of V. faba samples. The four equina accessions are separated from each other and form two distinct clades while the two major faba bean accessions are not unequivocally separated by the IRAP. Fluorescent In Situ Hybridization (FISH) analysis of sequences amplified by IRAP Athila revealed a wide distribution throughout V. faba chromosomes, confirming the whole-genome coverage of this molecular marker. Morphological characteristics were also assessed through cluster analysis of seed characters using the unweighted pair group method arithmetic average (UPGMA) and principal component analysis (PCA), showing a clear discrimination between faba bean major and equina groups. It was also found that the seed character most relevant to distinguish accessions was 100 seed weight. Seed morphological traits and IRAP evaluation give similar results supporting the potential of IRAP analysis for genetic diversity studies. View Full-Text
Keywords: faba bean locally adapted populations; genomic diversity; IRAP faba bean locally adapted populations; genomic diversity; IRAP
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Tomás, D.; Dias, A.L.; Silva, M.; Oliveira, H.R.; Suso, M.J.; Viegas, W.; Veloso, M.M. Genetic Diversity Assessment of Portuguese Cultivated Vicia faba L. through IRAP Markers. Diversity 2016, 8, 8.

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