Diversity 2010, 2(4), 439-449; doi:10.3390/d2040439
Article

Genetic Variability of Macedonian Tobacco Varieties Determined by Microsatellite Marker Analysis

Katarina Davalieva 1 email, Ivana Maleva 1 email, Kiril Filiposki 2 email, Ognen Spiroski 1 email and Georgi D. Efremov 1,* email
1 Research Centre for Genetic Engineering and Biotechnology, Macedonian Academy of Sciences and Arts, Krste Misirkov 2, POB 428, 1000 Skopje, Republic of Macedonia 2 University “St Kliment Ohridski”, Bitola, Tobacco Institute, Kicevski pat bb, 7500 Prilep, Republic of Macedonia
* Author to whom correspondence should be addressed.
Received: 20 January 2010; in revised form: 4 February 2010 / Accepted: 2 March 2010 / Published: 24 March 2010
(This article belongs to the Special Issue Assessment of Plant Genetic Diversity)
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Abstract: Tobacco (Nicotiana tabacum L.) is an important agricultural crop plant for the economy of many countries. Assessment of the genetic diversity of cultivated tobacco varieties is of importance for long-term tobacco improvement. Microsatellite markers are currently the marker system of choice for genetic analysis of allopolyploid plants. In this study, we evaluated the use of 30 microsatellite markers for identification of 10 varieties of cultivated tobacco in the Republic of Macedonia. We found 24 of the microsatellite markers to be polymorphic and sufficient for identification of these varieties. Cluster analysis showed that Macedonian tobacco varieties are classifiable into three distinct groups.
Keywords: Nicotiana tabacum L; PCR; microsatellite markers; tobacco varieties; genetic diversity

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MDPI and ACS Style

Davalieva, K.; Maleva, I.; Filiposki, K.; Spiroski, O.; Efremov, G.D. Genetic Variability of Macedonian Tobacco Varieties Determined by Microsatellite Marker Analysis. Diversity 2010, 2, 439-449.

AMA Style

Davalieva K, Maleva I, Filiposki K, Spiroski O, Efremov GD. Genetic Variability of Macedonian Tobacco Varieties Determined by Microsatellite Marker Analysis. Diversity. 2010; 2(4):439-449.

Chicago/Turabian Style

Davalieva, Katarina; Maleva, Ivana; Filiposki, Kiril; Spiroski, Ognen; Efremov, Georgi D. 2010. "Genetic Variability of Macedonian Tobacco Varieties Determined by Microsatellite Marker Analysis." Diversity 2, no. 4: 439-449.

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