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Int. J. Mol. Sci. 2008, 9(8), 1504-1514; doi:10.3390/ijms9081504
Article

TFIP11, CCNL1 and EWSR1 Protein-protein Interactions, and Their Nuclear Localization

, ,  and *
University of Southern California, School of Dentistry, Center for Craniofacial Molecular Biology, 2250 Alcazar Street, CSA room 103, Los Angeles, California 90033-1004, USA
* Author to whom correspondence should be addressed.
Received: 22 May 2008 / Revised: 14 August 2008 / Accepted: 15 August 2008 / Published: 25 August 2008
(This article belongs to the Section Bioinorganic Chemistry)
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Abstract

Previous studies using the yeast two-hybrid assay (Y2H) have identified cyclin L1 (CCNL1) and Ewing sarcoma breakpoint region 1 protein (EWSR1) as being interacting partners of tuftelin-interacting protein 11 (TFIP11). All three proteins are functionally related to the spliceosome and involved in pre-mRNA splicing activities. The spliceosome is a dynamic ribonucleoprotein complex responsible for pre-mRNA splicing of intronic regions, and is composed of five small nuclear RNAs (snRNAs) and ~140 proteins. TFIP11 appears to play a role in spliceosome disassembly allowing for the release of the bound lariat-intron. The roles of CCNL1 and EWSR1 in the spliceosome are poorly understood. Using fluorescently-tagged proteins and confocal microscopy we show that TFIP11, CCNL1 and EWSR1 frequently co-localize to speckled nuclear domains. These data would suggest that all three proteins participate in a common cellular activity related to RNA splicing events.
Keywords: Cyclin L1; Ewing’s sarcoma protein; nuclear speckles; pre-mRNA splicing; spliceosomal component 35; spliceosome; tuftelin-interacting protein 11. Cyclin L1; Ewing’s sarcoma protein; nuclear speckles; pre-mRNA splicing; spliceosomal component 35; spliceosome; tuftelin-interacting protein 11.
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Tannukit, S.; Wen, X.; Wang, H.; Paine, M.L. TFIP11, CCNL1 and EWSR1 Protein-protein Interactions, and Their Nuclear Localization. Int. J. Mol. Sci. 2008, 9, 1504-1514.

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