Redox Behavior of Anticancer Chalcone on a Glassy Carbon Electrode and Evaluation of its Interaction Parameters with DNA

Abstract: The interaction of anticancer chalcone [AMC, 1-(4'-aminophenyl)-3-(4-N,Ndimethylphenyl)- 2-propen-1-one] with DNA has been explored using electrochemical, spectroscopic and viscometric techniques. A shift in peak potential and decrease in peak current were observed in cyclic voltammetry and hypochromism accompanied with bathochromic shift were noticed in UV-Vis absorption spectroscopy. These findings were taken as evidence for AMC –DNA intercalation. A binding constant (K) with a value of 6.15 × 10⁵ M^-1 was obtained from CV data, which was also confirmed by UV-Vis absorption titration. Moreover, the diffusion coefficient of the drug with and without DNA (D_b and D_u), heterogeneous electron transfer rate constant (k_^o) and electron affinity (A) were also calculated from electrochemical data.