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Int. J. Mol. Sci. 2008, 9(5), 736-750; doi:10.3390/ijms9050736

Purification and Preliminary Crystallographic Analysis of a New Lys49-PLA2 from B. Jararacussu

Laboratório de Biologia Estrutural e Cristalografia, Instituto de Química, Universidade Estadual de Campinas, CP 6154, 13083-970, Campinas-SP, Brazil
Instituto de Biologia, Universidade Estadual de Campinas, Campinas-SP, Brazil
Laboratório de Química de Macromoléculas, UNESP/CLP, São Vicente-SP, Brazil
Author to whom correspondence should be addressed.
Received: 4 February 2008 / Revised: 6 March 2008 / Accepted: 22 March 2008 / Published: 8 May 2008
(This article belongs to the Special Issue Protein Crystallography)
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BjVIII is a new myotoxic Lys49-PLA2 isolated from Bothrops jararacussu venom that exhibits atypical effects on human platelet aggregation. To better understand the mode of action of BjVIII, crystallographic studies were initiated. Two crystal forms were obtained, both containing two molecules in the asymmetric unit (ASU). Synchrotron radiation diffraction data were collected to 2.0 °A resolution and 1.9 °A resolution for crystals belonging to the space group P212121 (a = 48:4 A° , b = 65:3 A° , c = 84:3 A° ) and space group P3121 (a = b = 55:7 A° , c = 127:9 A° ), respectively. Refinement is currently in progress and the refined structures are expected to shed light on the unusual platelet aggregation activity observed for BjVIII. View Full-Text
Keywords: Lys49-PLA2; Bothrops jararacussu; platelet aggregation; phospholipase crystallographic analysis Lys49-PLA2; Bothrops jararacussu; platelet aggregation; phospholipase crystallographic analysis

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Dos Santos, M.L.; Fagundes, F.H.R.; Teixeira, B.R.F.; Toyama, M.H.; Aparicio, R. Purification and Preliminary Crystallographic Analysis of a New Lys49-PLA2 from B. Jararacussu. Int. J. Mol. Sci. 2008, 9, 736-750.

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