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Int. J. Mol. Sci. 2008, 9(4), 571-577; doi:10.3390/ijms9040571

Extraction of Glycyrrhizic Acid and Glabridin from Licorice

,  and *
Center for Advanced Bioseperation Technology, Department of Chemical Engineering, Inha University, Incheon 402-751, Korea
* Author to whom correspondence should be addressed.
Received: 25 January 2008 / Revised: 18 February 2008 / Accepted: 12 March 2008 / Published: 16 April 2008
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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The extraction and separation conditions of glycyrrhizic acid and glabridin from licorice were investigated. By changing the different extraction solvents, procedures, times and temperature, the optimum extraction condition was established: the used of ethanol/water (30:70, v/v) as an extraction solvent, and 60 min dipping time under 50°C. The extracts of licorice were separated and determined by reversed-phase high performance liquid chromatography with a methanol/water (70:30, v/v, containing 1% acetic acid) as the mobile phase. Under the optimum extraction condition, 2.39 mg/g of glycyrrhizic acid and 0.92 mg/g of glabridin were extracted from Chinese licorice and the recoveries were 89.7% and 72.5% respectively.
Keywords: glycyrrhizic acid; glabridin; licorice; extraction; high-performance liquid chromatography glycyrrhizic acid; glabridin; licorice; extraction; high-performance liquid chromatography
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Tian, M.; Yan, H.; Row, K.H. Extraction of Glycyrrhizic Acid and Glabridin from Licorice. Int. J. Mol. Sci. 2008, 9, 571-577.

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