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Measurement of Antioxidant Activity of Wine Catechins, Procyanidins, Anthocyanins and Pyranoanthocyanins
Department of Pharmaceutics, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Palackého 1-3, 612 42 Brno, Czech Republic
Unidad de Nutricion y Bromatologia, University of Salamanca, Campus Miguel de Unamuno, 37007 Salamanca, Spain
Department of Natural Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Palackého 1-3, 612 42 Brno, Czech Republic
* Author to whom correspondence should be addressed.
Received: 13 June 2007; in revised form: 7 August 2007 / Accepted: 9 August 2007 / Published: 14 August 2007
Abstract: Nowadays, there is considerable interest in finding out about antioxidants thatare consumed in the habitual diet. It is known that polyphenols are involved in reducing therisk of diseases associated with oxidative stress. The in vitro antioxidant activity of theprincipal wine polyphenolic compounds (catechins, procyanidins, anthocyanins andpyranoanthocyanins) was studied in this work. Four distinct methods were used to assessthe antioxidant capacity of the tested compounds: inhibition of peroxynitrite mediatedtyrosine nitration, TEAC (Trolox equivalent antioxidant capacity assay), FRAP (Ferricreducing/antioxidant power assay) and TBARS (thiobarbituric acid reactive substances)methods. In general, it could be concluded that procyanidins were, among the in vitrotested groups, the ones which showed more antioxidant capacity using the four differentmethods, followed by anthocyanins and pyranoanthocyanins. On the basis of the simpleregression testing, there was a statistically significant relationship between these differentmethods used in aqueous phase (r > 0.92). However, no correlation was found between theresults obtained in lipid media with the TBARS method and those obtained in the aqueousmedia (peroxynitrite scavenging activity, TEAC and FRAP methods).
Keywords: Antioxidant activity; TEAC; TBARS; FRAP; peroxynitrite; procyanidins; anthocyanins; pyranoanthocyanins.
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Muselík, J.; García-Alonso, M.; Martín-López, M.P.; Žemlička, M.; Rivas-Gonzalo, J.C. Measurement of Antioxidant Activity of Wine Catechins, Procyanidins, Anthocyanins and Pyranoanthocyanins. Int. J. Mol. Sci. 2007, 8, 797-809.
Muselík J, García-Alonso M, Martín-López MP, Žemlička M, Rivas-Gonzalo JC. Measurement of Antioxidant Activity of Wine Catechins, Procyanidins, Anthocyanins and Pyranoanthocyanins. International Journal of Molecular Sciences. 2007; 8(8):797-809.
Muselík, Jan; García-Alonso, María; Martín-López, María P.; Žemlička, Milan; Rivas-Gonzalo, Julián C. 2007. "Measurement of Antioxidant Activity of Wine Catechins, Procyanidins, Anthocyanins and Pyranoanthocyanins." Int. J. Mol. Sci. 8, no. 8: 797-809.