Next Article in Journal
UVB-Protective Effects of Isoflavone Extracts from Soybean Cake in Human Keratinocytes
Previous Article in Journal
Catalytic Ring Hydrogenation of Benzoic Acid with Supported Transition Metal Catalysts in scCO2
Article Menu

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2007, 8(7), 635-650; doi:10.3390/i8070635

Variation of the Nuclear, Subnuclear and Chromosomal Flavanol Deposition in Hemlock and Rye

1
Department for Plant Science, Center of Life Science Weihenstephan (WZW), Technical University Munich (TUM), D-85354 Freising, Germany
2
Physical Biochemistry, Research Department Biosciences, WZW, TU Munich (TUM), D-85354 Freising, Germany
*
Author to whom correspondence should be addressed.
Received: 27 April 2007 / Revised: 31 May 2007 / Accepted: 27 June 2007 / Published: 10 July 2007
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
View Full-Text   |   Download PDF [535 KB, uploaded 19 June 2014]   |  

Abstract

Nuclei of hemlock (Tsuga canadensis and Tsuga canadensis var. nana) wereinvestigated for the presence of flavanols. Histochemical staining with p-dimethylaminocinnamaldehyde proved to be a highly valuable method yielding a brightblue flavanol coloration for nuclei. There was a significant variation in flavanol deposition(1) among nuclei, (2) at the subnuclear level and also (3) along the chromosomes duringmitosis. The presence of flavanols in nucleoli could not be established probably becausethey were too small, measuring less than 1 μm in diameter. In contrast to Tsuga, the cellsand nuclei of rootlets from rye (Secale cereale) were totally devoid of natural flavanols.However, externally added flavanols, catechin and epicatechin, were bound to the ryenuclei, while the rather large nucleoli failed to associate with the flavanols. The strong sinkactivity of nucleoplasm and chromosomes for flavanols in Tsuga and Secale indicates aprocess which is apparently widespread even in distantly related plant species. Variationsin chromatin-associated flavanols could to some extent be induced byacetylation/deacetylation of histones, as confirmed in the present study by means of UV-VIS spectroscopic titrations of histone sulphate and chemically acetylated histone sulphate.
Keywords: plant nuclei; flavanols; DMACA; histology; acetylated histones. plant nuclei; flavanols; DMACA; histology; acetylated histones.
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Feucht, W.; Dithmar, H.; Polster, J. Variation of the Nuclear, Subnuclear and Chromosomal Flavanol Deposition in Hemlock and Rye. Int. J. Mol. Sci. 2007, 8, 635-650.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top