Int. J. Mol. Sci. 2007, 8(12), 1225-1233; doi:10.3390/i8121233
Article

The Interaction of Sheep Genomic DNA with a Cobalt(II) Complex Containing p-Nitrobenzoate and N,N`-Diethylnicotinamide Ligands

Ali Arslantas 1,* email, A. Kadir Devrim 2  and Hacali Necefoglu 1
1 Department of Chemistry, Faculty of Arts and Sciences, Kafkas University, 36300 Kars, Turkey 2 Department of Biochemistry, Faculty of Veterinary Medicine, Kafkas University, Kars, Turkey
* Author to whom correspondence should be addressed.
Received: 15 May 2007; in revised form: 16 November 2007 / Accepted: 23 November 2007 / Published: 13 December 2007
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
PDF Full-text Download PDF Full-Text [220 KB, uploaded 20 June 2008 17:29 CEST]
Abstract: The synthesized cobalt(II) complex, CoPNBDENA and the binding of thiscomplex with sheep genomic DNA were investigated by UV–Visible absorption andviscosity techniques. Also the interaction of sheep genomic DNA with the complex wasstudied using the agarose gel electrophoresis method. The results indicated that thecomplex interacted with DNA. The nature of the binding seemed to be mainly anelectrostatic interaction between DNA and the cobalt(II) complex. Other binding modessuch as hydrogen bonds may also exist in this system. In this study, after the interaction ofDNA– CoPNBDENA, it was observed that the migration of the DNA band became slow asthe amount of cobalt(II) complex was increased. This clearly demonstrates that theCoPNBDENA complex neutralizes the negative charges of DNA.
Keywords: DNA-metal interaction; Cobalt(II) complex; Sheep genomic DNA; pnitrobenzoate; N; N`-Diethylnicotinamid

Article Statistics

Click here to load and display the download statistics.

Cite This Article

MDPI and ACS Style

Arslantas, A.; Devrim, A.K.; Necefoglu, H. The Interaction of Sheep Genomic DNA with a Cobalt(II) Complex Containing p-Nitrobenzoate and N,N`-Diethylnicotinamide Ligands. Int. J. Mol. Sci. 2007, 8, 1225-1233.

AMA Style

Arslantas A, Devrim AK, Necefoglu H. The Interaction of Sheep Genomic DNA with a Cobalt(II) Complex Containing p-Nitrobenzoate and N,N`-Diethylnicotinamide Ligands. International Journal of Molecular Sciences. 2007; 8(12):1225-1233.

Chicago/Turabian Style

Arslantas, Ali; Devrim, A. K.; Necefoglu, Hacali. 2007. "The Interaction of Sheep Genomic DNA with a Cobalt(II) Complex Containing p-Nitrobenzoate and N,N`-Diethylnicotinamide Ligands." Int. J. Mol. Sci. 8, no. 12: 1225-1233.

Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert