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Int. J. Mol. Sci. 2007, 8(12), 1225-1233; doi:10.3390/i8121233
Article

The Interaction of Sheep Genomic DNA with a Cobalt(II) Complex Containing p-Nitrobenzoate and N,N`-Diethylnicotinamide Ligands

1,* , 2 and 1
Received: 15 May 2007 / Revised: 16 November 2007 / Accepted: 23 November 2007 / Published: 13 December 2007
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

The synthesized cobalt(II) complex, CoPNBDENA and the binding of thiscomplex with sheep genomic DNA were investigated by UV–Visible absorption andviscosity techniques. Also the interaction of sheep genomic DNA with the complex wasstudied using the agarose gel electrophoresis method. The results indicated that thecomplex interacted with DNA. The nature of the binding seemed to be mainly anelectrostatic interaction between DNA and the cobalt(II) complex. Other binding modessuch as hydrogen bonds may also exist in this system. In this study, after the interaction ofDNA– CoPNBDENA, it was observed that the migration of the DNA band became slow asthe amount of cobalt(II) complex was increased. This clearly demonstrates that theCoPNBDENA complex neutralizes the negative charges of DNA.
Keywords: DNA-metal interaction; Cobalt(II) complex; Sheep genomic DNA; pnitrobenzoate; N; N`-Diethylnicotinamid DNA-metal interaction; Cobalt(II) complex; Sheep genomic DNA; pnitrobenzoate; N; N`-Diethylnicotinamid
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Arslantas, A.; Devrim, A.K.; Necefoglu, H. The Interaction of Sheep Genomic DNA with a Cobalt(II) Complex Containing p-Nitrobenzoate and N,N`-Diethylnicotinamide Ligands. Int. J. Mol. Sci. 2007, 8, 1225-1233.

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Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert