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Int. J. Mol. Sci., Volume 8, Issue 10 (October 2007) – 4 articles , Pages 1013-1063

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2578 KiB  
Article
Crystallization and Characterization of Galdieria sulphuraria RUBISCO in Two Crystal Forms: Structural Phase Transition Observed in P21 Crystal Form
by Michael Baranowski and Boguslaw Stec
Int. J. Mol. Sci. 2007, 8(10), 1039-1051; https://doi.org/10.3390/i8101039 - 23 Oct 2007
Cited by 4 | Viewed by 9040
Abstract
We have isolated ribulose-1,5-bisphosphate-carboxylase/oxygenase (RUBISCO)from the red algae Galdieria Sulphuraria. The protein crystallized in two different crystalforms, the I422 crystal form being obtained from high salt and the P21 crystal form beingobtained from lower concentration of salt and PEG. We report here the [...] Read more.
We have isolated ribulose-1,5-bisphosphate-carboxylase/oxygenase (RUBISCO)from the red algae Galdieria Sulphuraria. The protein crystallized in two different crystalforms, the I422 crystal form being obtained from high salt and the P21 crystal form beingobtained from lower concentration of salt and PEG. We report here the crystallization,preliminary stages of structure determination and the detection of the structural phasetransition in the P21 crystal form of G. sulphuraria RUBISCO. This red algae enzymebelongs to the hexadecameric class (L8S8) with an approximate molecular weight 0.6MDa.The phase transition in G. sulphuraria RUBISCO leads from two hexadecamers to a singlehexadecamer per asymmetric unit. The preservation of diffraction power in a phasetransition for such a large macromolecule is rare. Full article
(This article belongs to the Special Issue Protein Crystallography)
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939 KiB  
Article
Threading Cyclodextrins in Chloroform: A [2]Pseudorotaxane
by Giuseppe Gattuso, Claudia Gargiulli and Melchiorre F. Parisi
Int. J. Mol. Sci. 2007, 8(10), 1052-1063; https://doi.org/10.3390/i8101052 - 22 Oct 2007
Cited by 9 | Viewed by 9176
Abstract
The unusual self-assembly in chloroform of a novel cyclodextrin-based [2]pseudorotaxane, composed of heptakis-2,6-di-O-methyl-3-O-acetyl-β-cyclodextrin and a viologen-containing linear component, is reported. Full article
(This article belongs to the Section Molecular Recognition)
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208 KiB  
Article
Investigation of Phenolic Components of Hungarian Wines
by Péter Avar, Martin S. Pour Nikfardjam, Sándor Kunsági-Máté, Gergely Montskó, Zoltán Szabó, Katalin Böddi, Róbert Ohmacht and László Márk
Int. J. Mol. Sci. 2007, 8(10), 1028-1038; https://doi.org/10.3390/i8101028 - 29 Sep 2007
Cited by 25 | Viewed by 10659
Abstract
Ninety-two wines from the southernmost wine-producing region in Hungary(Villány) were analyzed for their polyphenolic content by high performance liquidchromatography (HPLC). Our results show that wine variety or vintage year could not bedistinguished based on polyphenol content, but winery origin could be. Resveratrolconcentration is [...] Read more.
Ninety-two wines from the southernmost wine-producing region in Hungary(Villány) were analyzed for their polyphenolic content by high performance liquidchromatography (HPLC). Our results show that wine variety or vintage year could not bedistinguished based on polyphenol content, but winery origin could be. Resveratrolconcentration is mainly dependent on variety and vintage year. The “human factor” (i.e.,winemaking style and technology) seems to be more decisive for the polyphenoliccomposition of red wines than other factors, such as variety and vintage year. Full article
(This article belongs to the Special Issue Phenolics and Polyphenolics)
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1485 KiB  
Article
Antioxidant Potential, Lipid Peroxidation Inhibition and Antimicrobial Activities of Satureja montana L. subsp. kitaibelii Extracts
by Gordana S. Ćetković, Jasna M. Čanadanović-Brunet, Sonja M. Djilas, Vesna T. Tumbas, Siniša L. Markov and Dragoljub D. Cvetković
Int. J. Mol. Sci. 2007, 8(10), 1013-1027; https://doi.org/10.3390/i8101013 - 29 Sep 2007
Cited by 82 | Viewed by 11125
Abstract
The antioxidant activity of different Satureja montana L. subsp. kitaibelii extracts was tested by measuring their ability to scavenge reactive hydroxyl radical during the Fenton reaction, using ESR spectroscopy. Also, the influence of these extracts on lipid peroxyl radicals obtained during lipid peroxidation [...] Read more.
The antioxidant activity of different Satureja montana L. subsp. kitaibelii extracts was tested by measuring their ability to scavenge reactive hydroxyl radical during the Fenton reaction, using ESR spectroscopy. Also, the influence of these extracts on lipid peroxyl radicals obtained during lipid peroxidation of: (I) sunflower oil (37°C, 3h) inducedby 4,4'-azobis(4-cyanovaleric acid) (ACVA) and (II) liposomes induced by 2,2'-azobis(2-amidino-propane)dihydrochloride (AAPH) was studied. n-Butanol extract had the bestantioxidant activity (100% at 0.5 mg/mL in Fenton reaction system; 89.21% at 5 mg/mL in system I; 83.38% at 5 mg/mL in system II). The antioxidant activities of the extracts significantly correlated with total phenolic content. The antimicrobial activity of Satureja montana L. subsp. kitaibelii extracts was investigated. Petroleum ether, chloroform and ethyl acetate extracts expressed a wide range of inhibiting activity against both gram-positive and gram-negative bacteria. Full article
(This article belongs to the Special Issue Phenolics and Polyphenolics)
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