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Isolation and Characterization of a Novel Four-Transmembrane Protein PMP22CD Specifically Expressed in the Testis
Department of Biochemistry and Molecular Biology, Institute of Biochemical Engineer, Medical School, Soochow University, Suzhou, P.R. China
School of Medical Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
Department of Histology and Embryology, Medical School, Soochow University, Suzhou, P.R. China
* Author to whom correspondence should be addressed.
Received: 26 September 2006; Accepted: 18 October 2006 / Published: 25 October 2006
Abstract: PMP22_Claudin family proteins play important roles in cell tight junction. In thisstudy, we have identified a novel member of this family, PMP22CD. Human PMP22CD wasfirst discovered by database sequence mining and analysis, and verified by cloning andsequencing. PMP22CD was isolated from the human testis cDNA library and mapped tochromosome 11q24.1 by browsing the UCSC genomic database. It contains an ORF with alength of 675bp, encoding a protein that contains a putative PMP22_Claudin domain withfour transmembrane helices. Its molecular weight and isoelectric point are predicted to be25.8kDa and 8.42, respectively. The PMP22CD protein is highly conservative in mammalanimals. Phylogenetic tree analysis indicated that PMP22CD stands for a new subgroup inthe PMP22/EMP/Claudin family. RT-PCR analysis showed that PMP22CD was specificallyexpressed in the testis. Green fluorescence protein localization analysis showed thatPMP22CD mainly surrounded the nuclear membrane, with a minority distribution in thecytoplasm. These results suggested that PMP22CD is a distant member of thePMP22/EMP/Claudin family and that it may have a novel function that does not involve celltight junction because it is not located at the cell membrane.
Keywords: PMP22_Claudin; Expression pattern; Testis; GFP localization
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Wang, M.; Luo, G.; Li, F.; Wu, S.; Jiang, J.; Huang, C. Isolation and Characterization of a Novel Four-Transmembrane Protein PMP22CD Specifically Expressed in the Testis. Int. J. Mol. Sci. 2006, 7, 425-437.
Wang M, Luo G, Li F, Wu S, Jiang J, Huang C. Isolation and Characterization of a Novel Four-Transmembrane Protein PMP22CD Specifically Expressed in the Testis. International Journal of Molecular Sciences. 2006; 7(10):425-437.
Wang, Minghua; Luo, Guanmei; Li, Fang; Wu, Shiliang; Jiang, Juxiang; Huang, Chaoqun. 2006. "Isolation and Characterization of a Novel Four-Transmembrane Protein PMP22CD Specifically Expressed in the Testis." Int. J. Mol. Sci. 7, no. 10: 425-437.