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Int. J. Mol. Sci. 2018, 19(9), 2712; https://doi.org/10.3390/ijms19092712

Extract of the Blood Circulation-Promoting Recipe-84 Can Protect Rat Retinas by Inhibiting the β-Catenin Signaling Pathway

1,†
,
1,2,†
,
3,* , 4
and
1,*
1
Department of Pharmacology, School of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan 430074, China
2
Chongqing Center for Drug Evaluation and Certification, Chongqing 400042, China
3
Key Laboratory of Chinese Internal Medicine of MOE and Beijing, Beijing University of Chinese Medicine, Beijing 100700, China
4
State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 31 July 2018 / Revised: 20 August 2018 / Accepted: 6 September 2018 / Published: 11 September 2018
(This article belongs to the Special Issue Nanomedicine/Molecular Medicine)
Full-Text   |   PDF [5946 KB, uploaded 14 September 2018]   |  

Abstract

Extract of the Blood Circulation-Promoting Recipe (EBR-84) from the Chinese Herbal medicine “Blood Circulation Promoting Recipe” could retard retinopathy development. This study investigated whether EBR-84 protects retinas by inhibiting the β-catenin pathway using a rat model of retinopathy and a retinal ganglion cell 5 (RGC-5) cell death model. RGC death was induced by either N-methyl-d-aspartic acid (NMDA) or TWS119 (an activator of the β-catenin pathway). After the corresponding treatment with EBR-84, RGC death and the protein expression levels of β-catenin, cyclooxygenase-2 (COX-2), and vascular endothelial growth factor (VEGF) in rat retinas were examined. β-Catenin accumulated in the retinal ganglion cell layer (GCL) of NMDA-treated rats. EBR-84 (3.9, 7.8, and 15.6 g/kg) significantly attenuated the NMDA-induced RGC loss accompanying the reduction of β-catenin expression. Moreover, the expression levels of COX-2 and VEGF were decreased by EBR-84 in a dose-dependent manner. For the TWS119-treated rats, EBR-84 also ameliorated RGC loss and lowered the expression levels of β-catenin, COX-2, and VEGF. In vitro, EBR-84 increased the viability of NMDA-treated RGC-5 while decreased β-catenin expression. In conclusion, EBR-84 retarded ratretinopathy, and the β-catenin signaling pathway played an important role during this protective process. View Full-Text
Keywords: EBR-84; retinopathy; NMDA; β-catenin; VEGF EBR-84; retinopathy; NMDA; β-catenin; VEGF
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Qin, Q.-F.; Liu, M.; Tian, G.-H.; Chen, J.; Li, Y.-S. Extract of the Blood Circulation-Promoting Recipe-84 Can Protect Rat Retinas by Inhibiting the β-Catenin Signaling Pathway. Int. J. Mol. Sci. 2018, 19, 2712.

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