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Int. J. Mol. Sci. 2018, 19(9), 2644; https://doi.org/10.3390/ijms19092644

Role of Ttca of Citrobacter Werkmanii in Bacterial Growth, Biocides Resistance, Biofilm Formation and Swimming Motility

Guangdong Open Laboratory of Applied Microbiology, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, State Key Laboratory of Applied Microbiology Southern China, Guangdong Institute of Microbiology, Guangzhou, Guangdong 510070, China
These two authors contribute equally to this paper.
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Received: 14 June 2018 / Revised: 28 August 2018 / Accepted: 30 August 2018 / Published: 6 September 2018
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

To screen, identify and study the genes involved in isothiazolone resistance and biofilm formation in Citrobacter werkmanii strain BF-6. A Tn5 transposon library of approximately 900 mutants of C. werkmanii strain BF-6 was generated and screened to isolate 1,2-benzisothiazolin-3-one (BIT) resistant strains. In addition, the tRNA 2-thiocytidine (32) synthetase gene (ttcA) was deleted through homologous recombination and the resulting phenotypic changes of the ΔttcA mutant were studied. A total of 3 genes were successfully identified, among which ΔttcA mutant exhibited a reduction in growth rate and swimming motility. On the other hand, an increase in biofilms formation in ΔttcA were observed but not with a significant resistance enhancement to BIT. This work, for the first time, highlights the role of ttcA gene of C. werkmanii strain BF-6 in BIT resistance and biofilm formation. View Full-Text
Keywords: Citrobacter werkmanii; biofilm formation; ttcA gene; Tn5 mutagenesis; swimming motility Citrobacter werkmanii; biofilm formation; ttcA gene; Tn5 mutagenesis; swimming motility
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Zhou, G.; Wang, Y.-S.; Peng, H.; Huang, X.-M.; Xie, X.-B.; Shi, Q.-S. Role of Ttca of Citrobacter Werkmanii in Bacterial Growth, Biocides Resistance, Biofilm Formation and Swimming Motility. Int. J. Mol. Sci. 2018, 19, 2644.

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