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Int. J. Mol. Sci. 2018, 19(8), 2209; https://doi.org/10.3390/ijms19082209

Effects of Lipid Saturation on the Surface Properties of Human Meibum Films

1
Department of Optics and Spectroscopy, Faculty of Physics, St. Kliment Ohridski University of Sofia, Sofia 1164, Bulgaria
2
Department of Ophthalmology and Visual Sciences, University of Louisville, Louisville, KY 40292, USA
3
Department of Cytology, Histology and Embryology, Faculty of Biology, St. Kliment Ohridski University of Sofia, Sofia 1164, Bulgaria
4
Department of Ophthalmology, Kyoto Prefectural University of Medicine, 602-8566 Kyoto, Japan
*
Author to whom correspondence should be addressed.
Received: 4 June 2018 / Revised: 23 July 2018 / Accepted: 24 July 2018 / Published: 28 July 2018
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Elevated levels of acyl chain saturation of meibomian lipids are associated with vastly different effects: from enhanced tear film (TF) stability in infants to shortened TF breakup time in meibomian gland disease patients. Thus it is important to study the effect of saturation on the surface properties of human meibum (MGS). Therefore, MGS films (1, 2, 3, 4, 5, 10, 25, 50, 67, and 100% saturation) were spread at the air/water interface of a Langmuir surface balance. The layers’ capability to reorganize during dynamic area changes was accessed via the surface pressure (π)-area (A) compression isotherms and step/relaxation dilatational rheology studies. Film structure was monitored with Brewster angle microscopy. The raise in the % (at ≥10%) of saturation resulted in the formation of stiffer, thicker, and more elastic films at π ≥ 12 mN/m with the effects being proportional to the saturation level. At the same time, at low (≤10 mN/m) π the raise in saturation resulted in altered spreading and heterogeneous structure of MGS layers. The strong impact of saturation on MGS surface properties correlates with our recent spectroscopy study, which demonstrated that saturation induced increase of MGS acyl chain order, phase transition temperature, and cooperativity. View Full-Text
Keywords: human meibum; acyl chain saturation; Langmuir films; tear film stability; surface properties human meibum; acyl chain saturation; Langmuir films; tear film stability; surface properties
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Nencheva, Y.; Ramasubramanian, A.; Eftimov, P.; Yokoi, N.; Borchman, D.; Georgiev, G.A. Effects of Lipid Saturation on the Surface Properties of Human Meibum Films. Int. J. Mol. Sci. 2018, 19, 2209.

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