Next Article in Journal
Phosphokinome Analysis of Barth Syndrome Lymphoblasts Identify Novel Targets in the Pathophysiology of the Disease
Next Article in Special Issue
Effects of Oat Bran on Nutrient Digestibility, Intestinal Microbiota, and Inflammatory Responses in the Hindgut of Growing Pigs
Previous Article in Journal
Olive Oil Nutraceuticals in the Prevention and Management of Diabetes: From Molecules to Lifestyle
Previous Article in Special Issue
Dietary Supplementation with Oleum Cinnamomi Improves Intestinal Functions in Piglets
Article Menu
Issue 7 (July) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2018, 19(7), 2025; https://doi.org/10.3390/ijms19072025

Allyl Isothiocyanate Ameliorates Dextran Sodium Sulfate-Induced Colitis in Mouse by Enhancing Tight Junction and Mucin Expression

1
Department of Anatomy and Cell Biology, College of Veterinary Medicine, Seoul National University, 08826 Seoul, Korea
2
Gachon Institute of Pharmaceutical Sciences, Gachon University, 21936 Incheon, Korea
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 18 June 2018 / Accepted: 9 July 2018 / Published: 12 July 2018
(This article belongs to the Special Issue Nutrition and Gut Health)
Full-Text   |   PDF [2065 KB, uploaded 12 July 2018]   |  

Abstract

Inflammatory bowel disease (IBD) is characterized by chronic or recurrent inflammation of the gastrointestinal tract. Even though the current strategies to treat IBD include anti-inflammatory drugs and immune modulators, these treatments have side-effects. New strategies are, therefore, required to overcome the limitations of the therapies. In this study, we investigated the anti-colitic effects of allyl isothiocyanate (AITC), which is an active ingredient present in Wasabia japonica. The DSS-induced colitis model in the mouse was used to mimic human IBD and we observed that AITC treatment ameliorated the severity of colitis. We further studied the mechanism involved to ameliorate the colitis. To investigate the involvement of AITC on the intestinal barrier function, the effect on the intercellular tight junction was evaluated in the Caco-2 cell line while mucin expression was assessed in the LS174T cell line. AITC positively regulated tight junction proteins and mucin 2 (MUC2) against DSS-induced damage or depletion. Our data of in vivo studies were also consistent with the in vitro results. Furthermore, we observed that MUC2 increased by AITC is dependent on ERK signaling. In conclusion, we propose that AITC can be considered as a new strategy for treating IBD by modulating tight junction proteins and mucin. View Full-Text
Keywords: allyl isothiocyanate (AITC); Wasabia japonica; intestinal epithelial barrier; tight junction; mucin; mucin 2 (MUC2); goblet cell; dextran sodium sulfate (DSS); colitis allyl isothiocyanate (AITC); Wasabia japonica; intestinal epithelial barrier; tight junction; mucin; mucin 2 (MUC2); goblet cell; dextran sodium sulfate (DSS); colitis
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Kim, M.W.; Choi, S.; Kim, S.Y.; Yoon, Y.S.; Kang, J.-H.; Oh, S.H. Allyl Isothiocyanate Ameliorates Dextran Sodium Sulfate-Induced Colitis in Mouse by Enhancing Tight Junction and Mucin Expression. Int. J. Mol. Sci. 2018, 19, 2025.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top