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Int. J. Mol. Sci. 2018, 19(7), 2009; https://doi.org/10.3390/ijms19072009

Isolation and Characterization of a Green-Tissue Promoter from Common Wild Rice (Oryza rufipogon Griff.)

1
MOA Key Laboratory on Safety Assessment (Molecular) of Agri-GMO, Institute of Biotechnology, Chinese Academy of Agricultural Sciences, Beijing 100081, China
2
College of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China
3
Experimental Center Basic Medical Teaching, Capital Medical University, Beijing 100069, China
*
Authors to whom correspondence should be addressed.
Received: 8 June 2018 / Revised: 28 June 2018 / Accepted: 5 July 2018 / Published: 10 July 2018
(This article belongs to the Special Issue Plant Genetics and Molecular Breeding)
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Abstract

Promoters play a very important role in the initiation and regulation of gene transcription. Green-tissue promoter is of great significance to the development of genetically modified crops. Based on RNA-seq data and RT-PCR expression analysis, this study screened a gene, OrGSE (GREEN SPECIAL EXPRESS), which is expressed specifically in green tissues. The study also isolated the promoter of the OrGSE gene (OrGSEp), and predicted many cis-acting elements, such as the CAAT-Box and TATA-Box, and light-responding elements, including circadian, G-BOX and GT1 CONSENSUS. Histochemical analysis and quantification of GUS activity in transgenic Arabidopsis thaliana plants expressing GUS under the control of OrGSEp revealed that this promoter is not only green tissue-specific, but also light-inducible. The ability of a series of 5’-deletion fragments of OrGSEp to drive GUS expression in Arabidopsis was also evaluated. We found that the promoter region from −54 to −114 is critical for the promoter function, and the region from −374 to −114 may contain core cis-elements involved in light response. In transgenic rice expressing GUS under the control of OrGSEp, visualization and quantification of GUS activity showed that GUS was preferentially expressed in green tissues and not in endosperm. OrGSEp is a useful regulatory element for breeding pest-resistant crops. View Full-Text
Keywords: common wild rice; Promoter; Green tissue-specific expression; light-induced common wild rice; Promoter; Green tissue-specific expression; light-induced
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Xue, M.; Long, Y.; Zhao, Z.; Huang, G.; Huang, K.; Zhang, T.; Jiang, Y.; Yuan, Q.; Pei, X. Isolation and Characterization of a Green-Tissue Promoter from Common Wild Rice (Oryza rufipogon Griff.). Int. J. Mol. Sci. 2018, 19, 2009.

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