Next Article in Journal
Meta-Analysis and Experimental Validation Identified FREM2 and SPRY1 as New Glioblastoma Marker Candidates
Next Article in Special Issue
Unconventional Imaging Methods to Capture Transient Structures during Actomyosin Interaction
Previous Article in Journal
Co-Administration of Melatonin Effectively Enhances the Therapeutic Effects of Pioglitazone on Mesenchymal Stem Cells Undergoing Indoxyl Sulfate-Induced Senescence through Modulation of Cellular Prion Protein Expression
Article Menu
Issue 5 (May) cover image

Export Article

Open AccessReview
Int. J. Mol. Sci. 2018, 19(5), 1368; https://doi.org/10.3390/ijms19051368

Electron Microscopic Recording of the Power and Recovery Strokes of Individual Myosin Heads Coupled with ATP Hydrolysis: Facts and Implications

1
Department of Physiology, School of Medicine, Teikyo University, Tokyo, Japan
2
Department of Integrated Sciences in Physics and Biology, College of Humanities and Science, Nihon University, Tokyo, Japan
*
Author to whom correspondence should be addressed.
Received: 6 February 2018 / Revised: 26 April 2018 / Accepted: 27 April 2018 / Published: 4 May 2018
(This article belongs to the Special Issue The Actin-Myosin Interaction in Muscle)
Full-Text   |   PDF [9637 KB, uploaded 4 May 2018]   |  

Abstract

The most straightforward way to get information on the performance of individual myosin heads producing muscle contraction may be to record their movement, coupled with ATP hydrolysis, electron-microscopically using the gas environmental chamber (EC). The EC enables us to visualize and record ATP-induced myosin head movement in hydrated skeletal muscle myosin filaments. When actin filaments are absent, myosin heads fluctuate around a definite neutral position, so that their time-averaged mean position remains unchanged. On application of ATP, myosin heads are found to move away from, but not towards, the bare region, indicating that myosin heads perform a recovery stroke (average amplitude, 6 nm). After exhaustion of ATP, myosin heads return to their neutral position. In the actin–myosin filament mixture, myosin heads form rigor actin myosin linkages, and on application of ATP, they perform a power stroke by stretching adjacent elastic structures because of a limited amount of applied ATP ≤ 10 µM. The average amplitude of the power stroke is 3.3 nm and 2.5 nm at the distal and the proximal regions of the myosin head catalytic domain (CAD), respectively. The power stroke amplitude increases appreciably at low ionic strength, which is known to enhance Ca2+-activated force in muscle. In both the power and recovery strokes, myosin heads return to their neutral position after exhaustion of ATP. View Full-Text
Keywords: myosin head power stroke; myosin head recovery stroke; muscle contraction; gas environmental chamber; myosin head neutral configuration myosin head power stroke; myosin head recovery stroke; muscle contraction; gas environmental chamber; myosin head neutral configuration
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Sugi, H.; Chaen, S.; Akimoto, T. Electron Microscopic Recording of the Power and Recovery Strokes of Individual Myosin Heads Coupled with ATP Hydrolysis: Facts and Implications. Int. J. Mol. Sci. 2018, 19, 1368.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top