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Int. J. Mol. Sci. 2018, 19(4), 1249; https://doi.org/10.3390/ijms19041249

Molecular Characterization and the Function of Argonaute3 in RNAi Pathway of Plutella xylostella

1
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China
2
Joint International Research Laboratory of Ecological Pest Control, Ministry of Education, Fuzhou 350002, China
3
Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of Agriculture, Fuzhou 350002, China
4
Key Laboratory of Green Pest Control (Fujian Agriculture and Forestry University), Fujian Province University, Fuzhou 350002, China
5
Key Laboratory of Natural Pesticides and Chemical Biology of the Ministry of Education, South China Agricultural University, Guangzhou 510642, China
6
Department of Biological Sciences, Brock University, 500 Glenridge Avenue, St. Catharines, ON L2S 3A1, Canada
*
Author to whom correspondence should be addressed.
Received: 9 March 2018 / Revised: 14 April 2018 / Accepted: 17 April 2018 / Published: 20 April 2018
(This article belongs to the Special Issue Molecular Entomology of Insects of Economic Importance)
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Abstract

Argonaute (Ago) protein family plays a key role in the RNA interference (RNAi) process in different insects including Lepidopteran. However, the role of Ago proteins in the RNAi pathway of Plutella xylostella is still unknown. We cloned an Argonaute3 gene in P. xylostella (PxAgo3) with the complete coding sequence of 2832 bp. The encoded protein had 935 amino acids with an expected molecular weight of 108.9 kDa and an isoelectric point of 9.29. It contained a PAZ (PIWI/Argonaute/Zwile) domain and PIWI (P-element-induced whimpy testes) domain. PxAgo3 was classified into the Piwi subfamily of Ago proteins with a high similarity of 93.0% with Bombyx mori Ago3 (BmAgo3). The suppression of PxAgo3 by dsPxAgo3 was observed 3 h after treatment and was maintained until 24 h. Knockdown of PxAgo3 decreased the suppression level of PxActin by dsPxActin in P. xylostella cells, while overexpression of PxAgo3 increased the RNAi efficiency. Our results suggest that PxAgo3 play a key role in the double stranded RNA (dsRNA)-regulated RNAi pathway in P. xylostella. View Full-Text
Keywords: lepidopteran pest; PxAgo3; phylogenetic tree; dsRNA; RNAi process lepidopteran pest; PxAgo3; phylogenetic tree; dsRNA; RNAi process
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Hameed, M.S.; Wang, Z.; Vasseur, L.; Yang, G. Molecular Characterization and the Function of Argonaute3 in RNAi Pathway of Plutella xylostella. Int. J. Mol. Sci. 2018, 19, 1249.

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