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Int. J. Mol. Sci. 2018, 19(3), 706; https://doi.org/10.3390/ijms19030706

Aged Mouse Cortical Microglia Display an Activation Profile Suggesting Immunotolerogenic Functions

1
Institute for Anatomy and Cell Biology, Department of Molecular Embryology, Faculty of Medicine, University of Freiburg, Freiburg 79104, Germany
2
Faculty of Biology, University of Freiburg, Freiburg 79104, Germany
3
Department of Anatomy and Histology, Faculty of Veterinary Medicine, Assiut University, Assiut 71526, Egypt
4
Institute of Anatomy, University of Rostock, Rostock 18057, Germany
*
Author to whom correspondence should be addressed.
Received: 31 January 2018 / Revised: 21 February 2018 / Accepted: 25 February 2018 / Published: 1 March 2018
(This article belongs to the Special Issue Molecular and Cellular Mechanisms of Aging and Age-Related Disorders)
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Abstract

Microglia are the resident immune cells of the central nervous system (CNS) and participate in physiological and pathological processes. Their unique developmental nature suggests age-dependent structural and functional impairments that might contribute to neurodegenerative diseases. In the present study, we addressed the age-dependent changes in cortical microglia gene expression patterns and the expression of M1- and M2-like activation markers. Iba1 immunohistochemistry, isolation of cortical microglia followed by fluorescence-activated cell sorting and RNA isolation to analyze transcriptional changes in aged cortical microglia was performed. We provide evidence that aging is associated with decreased numbers of cortical microglia and the establishment of a distinct microglia activation profile including upregulation of Ifi204, Lilrb4, Arhgap, Oas1a, Cd244 and Ildr2. Moreover, flow cytometry revealed that aged cortical microglia express increased levels of Cd206 and Cd36. The data presented in the current study indicate that aged mouse cortical microglia adopt a distinct activation profile, which suggests immunosuppressive and immuno-tolerogenic functions. View Full-Text
Keywords: microglia; aging; cerebral cortex; neuroinflammation microglia; aging; cerebral cortex; neuroinflammation
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Zöller, T.; Attaai, A.; Potru, P.S.; Ruß, T.; Spittau, B. Aged Mouse Cortical Microglia Display an Activation Profile Suggesting Immunotolerogenic Functions. Int. J. Mol. Sci. 2018, 19, 706.

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