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Int. J. Mol. Sci. 2018, 19(2), 636; https://doi.org/10.3390/ijms19020636

Phytochemical Analysis by HPLC–HRESI-MS and Anti-Inflammatory Activity of Tabernaemontana catharinensis

1
Laboratory of Pharmacognosy (PNBio), Department of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Rio Grande do Norte, Avenida General Gustavo Cordeiro de Farias, S/N, Petrópolis 59012-570, Natal, Brazil
2
Laboratory of Technology and Pharmaceutical Biotechnology (Tecbiofar), Department of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Rio Grande do Norte, Avenida General Gustavo Cordeiro de Farias, S/N, Petrópolis 59012-570, Natal, Brazil
3
Faculty of Health Sciences of Trairí, Federal University of Rio Grande do Norte, Avenida Trairí, S/N, Centro 59200-000, Santa Cruz, Brazil
4
Department of Biophysics and Pharmacology, Bioscience Center, Campus Universitário, Federal University of Rio Grande do Norte, Avenida Senador Salgado Filho, 3000, Lagoa Nova 59072-970, Natal, Brazil
5
Graduate Program of Biotechnology, Laureate Universities, Universidade Potiguar (UnP), Avenida Senador Salgado Filho, 1610, Lagoa Nova 59056-000, Natal, Brazil
*
Authors to whom correspondence should be addressed.
Received: 22 November 2017 / Revised: 7 January 2018 / Accepted: 13 January 2018 / Published: 24 February 2018
(This article belongs to the Section Bioactives and Nutraceuticals)
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Abstract

Tabernaemontana catharinensis (Apocynaceae) has been popularly used by folk medicine because of its anti-inflammatory, analgesic, and antiophidic properties. This study aims to analyze the flavonoids composition of the hydroethanolic extract and of the ethyl acetate (EtOAc) and butanol (BuOH) fractions of T. catharinensis leaves, as well as to evaluate their anti-inflammatory activity using in vivo models. The phytochemical profile, determined by High-Performance Liquid Chromatography–High-Resolution Electrospray Ionization-Mass Spectrometry (HPLC–HRESI-MS), showed the presence of flavonoids mainly having an isorhamnetin nucleus. The anti-inflammatory activity was evaluated in carrageenan-induced paw edema (pre- and post-treatment) with oral administration of a T. catharinensis hydroethanolic extract (50, 100, and 150 mg/kg) and of organic fractions (50 mg/kg). The extract and fractions showed antiedematogenic activity by decreasing myeloperoxidase (MPO) production. In the zymosan-air-pouch model, the extract and fractions inhibited leukocyte migration and significantly decreased the levels of various proteins, such as MPO, interleukin (IL)-1β, and tumor necrosis factor (TNF)-α. The cytotoxicity was evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, which revealed no cytotoxicity of the extract and the fractions. These results suggest that the hydroethanolic extract and organic fractions of T. catharinensis leaves have sufficient anti-inflammatory activity to support the popular use of this plant in the treatment of inflammatory disorders. View Full-Text
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Marques, J.I.; Alves, J.S.F.; Torres-Rêgo, M.; Furtado, A.A.; Siqueira, E.M.S.; Galinari, E.; Araújo, D.F.S.; Guerra, G.C.B.; Azevedo, E.P.; Fernandes-Pedrosa, M.F.; Zucolotto, S.M. Phytochemical Analysis by HPLC–HRESI-MS and Anti-Inflammatory Activity of Tabernaemontana catharinensis. Int. J. Mol. Sci. 2018, 19, 636.

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