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Int. J. Mol. Sci. 2018, 19(2), 494; https://doi.org/10.3390/ijms19020494

Consecutive Isoproterenol and Adenosine Treatment Confers Marked Protection against Reperfusion Injury in Adult but Not in Immature Heart: A Role for Glycogen

1
Bristol Medical School, University of Bristol, Bristol BS8 1TH, UK
2
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Comenius University in Bratislava, 814 99 Bratislava, Slovakia
3
School of Biochemistry, University of Bristol, Bristol BS8 1TH, UK
4
School of Physiology, Pharmacology & Neuroscience, University of Bristol, Bristol BS8 1TH, UK
*
Author to whom correspondence should be addressed.
Received: 20 December 2017 / Revised: 2 February 2018 / Accepted: 2 February 2018 / Published: 7 February 2018
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Abstract

Consecutive treatment of adult rat heart with isoproterenol and adenosine (Iso/Aden), known to consecutively activate PKA/PKC signaling, is cardioprotective against ischemia and reperfusion (I/R). Whether this is cardioprotective in an immature heart is unknown. Langendorff–perfused hearts from adult and immature (60 and 14 days old) male Wistar rats were exposed to 30 min ischemia and 120 min reperfusion, with or without prior perfusion with 5 nM Iso for 3 min followed by 30 μM Aden for 5 min. Changes in hemodynamics (developed pressure and coronary flow) and cardiac injury (Lactate Dehydrogenase (LDH) release and infarct size) were measured. Additional hearts were used to measure glycogen content. Iso induced a similar inotropic response in both age groups. Treatment with Iso/Aden resulted in a significant reduction in time to the onset of ischemic contracture in both age groups whilst time to peak contracture was significantly shorter only in immature hearts. Upon reperfusion, the intervention reduced cardiac injury and functional impairment in adults with no protection of immature heart. Immature hearts have significantly less glycogen content compared to adult. This work shows that Iso/Aden perfusion confers protection in an adult heart but not in an immature heart. It is likely that metabolic differences including glycogen content contribute to this difference. View Full-Text
Keywords: ischemia; isoproterenol; adenosine; cAMP; immature heart; cardioprotection; glycogen ischemia; isoproterenol; adenosine; cAMP; immature heart; cardioprotection; glycogen
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Lewis, M.; Szobi, A.; Balaska, D.; Khaliulin, I.; Adameova, A.; Griffiths, E.; Orchard, C.H.; Suleiman, M.-S. Consecutive Isoproterenol and Adenosine Treatment Confers Marked Protection against Reperfusion Injury in Adult but Not in Immature Heart: A Role for Glycogen. Int. J. Mol. Sci. 2018, 19, 494.

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