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Int. J. Mol. Sci. 2018, 19(2), 391; https://doi.org/10.3390/ijms19020391

S1P Signalling Differentially Affects Migration of Peritoneal B Cell Populations In Vitro and Influences the Production of Intestinal IgA In Vivo

1
Department of General Surgery, Visceral, Thoracic and Vascular Surgery, Surgery, Universitätsmedizin Greifswald, Greifswald 17475, Germany
2
Max-Delbrück-Centre for Molecular Medicine, Berlin 13125, Germany
*
Author to whom correspondence should be addressed.
Received: 5 December 2017 / Revised: 15 January 2018 / Accepted: 24 January 2018 / Published: 29 January 2018
(This article belongs to the Special Issue Sphingolipids: Signals and Disease)
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Abstract

Introduction: Sphingosine-1-phosphate (S1P) regulates the migration of follicular B cells (B2 cells) and directs the positioning of Marginal zone B cells (MZ B cells) within the spleen. The function of S1P signalling in the third B cell lineage, B1 B cells, mainly present in the pleural and peritoneal cavity, has not yet been determined. Methods: S1P receptor expression was analysed in peritoneal B cells by real-time polymerase chain reaction (qPCR). The chemotactic response to S1P was studied in vitro. The role of S1P signalling was further explored in a s1p4−/− mouse strain. Results: Peritoneal B cells expressed considerable amounts of the S1P receptors 1 and 4 (S1P1 and S1P4, respectively). S1P1 showed differential expression between the distinct peritoneal B cell lineages. While B2 cells showed no chemotactic response to S1P, B1 B cells showed a migration response to S1P. s1p4−/− mice displayed significant alterations in the composition of peritoneal B cell populations, as well as a significant reduction of mucosal immunoglobulin A (IgA) in the gut. Discussion: S1P signalling influences peritoneal B1 B cell migration. S1P4 deficiency alters the composition of peritoneal B cell populations and reduces secretory IgA levels. These findings suggest that S1P signalling may be a target to modulate B cell function in inflammatory intestinal pathologies. View Full-Text
Keywords: peritoneal B cells; B1 B cells; sphingosine-1-phosphate (S1P); mucosal immunity; lymphocyte trafficking peritoneal B cells; B1 B cells; sphingosine-1-phosphate (S1P); mucosal immunity; lymphocyte trafficking
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Kleinwort, A.; Lührs, F.; Heidecke, C.-D.; Lipp, M.; Schulze, T. S1P Signalling Differentially Affects Migration of Peritoneal B Cell Populations In Vitro and Influences the Production of Intestinal IgA In Vivo. Int. J. Mol. Sci. 2018, 19, 391.

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