Deciphering the Functional Composition of Fusogenic Liposomes
AbstractCationic liposomes are frequently used as carrier particles for nucleic acid delivery. The most popular formulation is the equimolar mixture of two components, a cationic lipid and a neutral phosphoethanolamine. Its uptake pathway has been described as endocytosis. The presence of an aromatic molecule as a third component strongly influences the cellular uptake process and results in complete membrane fusion instead of endocytosis. Here, we systematically varied all three components of this lipid mixture and determined how efficiently the resulting particles fused with the plasma membrane of living mammalian cells. Our results show that an aromatic molecule and a cationic lipid component with conical molecular shape are essential for efficient fusion induction. While a neutral lipid is not mandatory, it can be used to control fusion efficiency and, in the most extreme case, to revert the uptake mechanism back to endocytosis. View Full-Text
- Supplementary File 1:
PDF-Document (PDF, 1821 KB)
Scifeed alert for new publicationsNever miss any articles matching your research from any publisher
- Get alerts for new papers matching your research
- Find out the new papers from selected authors
- Updated daily for 49'000+ journals and 6000+ publishers
- Define your Scifeed now
Kolašinac, R.; Kleusch, C.; Braun, T.; Merkel, R.; Csiszár, A. Deciphering the Functional Composition of Fusogenic Liposomes. Int. J. Mol. Sci. 2018, 19, 346.
Kolašinac R, Kleusch C, Braun T, Merkel R, Csiszár A. Deciphering the Functional Composition of Fusogenic Liposomes. International Journal of Molecular Sciences. 2018; 19(2):346.Chicago/Turabian Style
Kolašinac, Rejhana; Kleusch, Christian; Braun, Tobias; Merkel, Rudolf; Csiszár, Agnes. 2018. "Deciphering the Functional Composition of Fusogenic Liposomes." Int. J. Mol. Sci. 19, no. 2: 346.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.