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Int. J. Mol. Sci. 2018, 19(1), 84; https://doi.org/10.3390/ijms19010084

Transcriptomic Profiling and Physiological Analysis of Haloxylon ammodendron in Response to Osmotic Stress

1
State Key Laboratory of Grassland Agro-Ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China
2
College of Agriculture and Forestry, Longdong University, Qingyang 745000, China
3
College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 23 November 2017 / Revised: 23 December 2017 / Accepted: 25 December 2017 / Published: 29 December 2017
(This article belongs to the Section Molecular Plant Sciences)
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Abstract

Haloxylon ammodendron, a perennial xero-halophyte, is an essential species for investigating the effects of drought on desert tree. To gain a comprehensive knowledge on the responses of H. ammodendron to drought stress, we specially performed the molecular and physiological analysis of H. ammodendron in response to −0.75 MPa osmotic stress for six and 24 h in lab condition via RNA-seq and digital gene expression (DGE). In total, 87,109 unigenes with a mean length of 680 bp and 13,486 potential simple sequence repeats (SSRs) were generated, and 3353 differentially expressed genes (DEGs) in shoots and 4564 in roots were identified under stress. These DEGs were mainly related to ion transporters, signal transduction, ROS-scavenging, photosynthesis, cell wall organization, membrane stabilization and hormones. Moreover, the physiological changes of inorganic ions and organic solute content, peroxidase (POD) activity and osmotic potential were in accordance with dynamic transcript profiles of the relevant genes. In this study, a detailed investigation of the pathways and candidate genes identified promote the research on the molecular mechanisms of abiotic stress tolerance in the xero-halophytic species. Our data provides valuable genetic resources for future improvement of forage and crop species for better adaptation to abiotic stresses. View Full-Text
Keywords: Haloxylon ammodendron; osmotic stress; molecular responses; regulation pathways; physiological analysis; osmoprotectants Haloxylon ammodendron; osmotic stress; molecular responses; regulation pathways; physiological analysis; osmoprotectants
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Gao, H.-J.; Lü, X.-P.; Zhang, L.; Qiao, Y.; Zhao, Q.; Wang, Y.-P.; Li, M.-F.; Zhang, J.-L. Transcriptomic Profiling and Physiological Analysis of Haloxylon ammodendron in Response to Osmotic Stress. Int. J. Mol. Sci. 2018, 19, 84.

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