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Int. J. Mol. Sci. 2018, 19(1), 181; https://doi.org/10.3390/ijms19010181

Halfway between 2D and Animal Models: Are 3D Cultures the Ideal Tool to Study Cancer-Microenvironment Interactions?

1
Stem Cell and Microenvironment Laboratory, Weill Cornell Medical College in Qatar, Qatar Foundation, Education City, Doha 24144, Qatar
2
Department of Genetic Medicine, Weill Cornell Medical College, New York, NY 10065, USA
3
UMR INSERM U965, Angiogenèse et Recherche Translationnelle, Hôpital Lariboisière, 49 bd de la Chapelle, 75010 Paris, France
4
Service de Gynécologie-Obstétrique et Médecine de la Reproduction, Centre Hospitalier Intercommunal de Créteil, Faculté de Médecine de Créteil UPEC, Paris XII, 40 Avenue de Verdun, 94000 Créteil, France
5
INSERM U955, Equipe 7, 94000 Créteil, France
*
Author to whom correspondence should be addressed.
Received: 27 November 2017 / Revised: 3 January 2018 / Accepted: 4 January 2018 / Published: 18 January 2018
(This article belongs to the Special Issue Tumor Microenvironment)
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Abstract

An area that has come to be of tremendous interest in tumor research in the last decade is the role of the microenvironment in the biology of neoplastic diseases. The tumor microenvironment (TME) comprises various cells that are collectively important for normal tissue homeostasis as well as tumor progression or regression. Seminal studies have demonstrated the role of the dialogue between cancer cells (at many sites) and the cellular component of the microenvironment in tumor progression, metastasis, and resistance to treatment. Using an appropriate system of microenvironment and tumor culture is the first step towards a better understanding of the complex interaction between cancer cells and their surroundings. Three-dimensional (3D) models have been widely described recently. However, while it is claimed that they can bridge the gap between in vitro and in vivo, it is sometimes hard to decipher their advantage or limitation compared to classical two-dimensional (2D) cultures, especially given the broad number of techniques used. We present here a comprehensive review of the different 3D methods developed recently, and, secondly, we discuss the pros and cons of 3D culture compared to 2D when studying interactions between cancer cells and their microenvironment. View Full-Text
Keywords: tumor microenvironment; 3D culture; 3D anchorage independent culture; 2D culture; tumor proliferation; tumor migration; chemoresistance tumor microenvironment; 3D culture; 3D anchorage independent culture; 2D culture; tumor proliferation; tumor migration; chemoresistance
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Hoarau-Véchot, J.; Rafii, A.; Touboul, C.; Pasquier, J. Halfway between 2D and Animal Models: Are 3D Cultures the Ideal Tool to Study Cancer-Microenvironment Interactions? Int. J. Mol. Sci. 2018, 19, 181.

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