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Int. J. Mol. Sci. 2018, 19(1), 158; https://doi.org/10.3390/ijms19010158

A High-Resolution Proteomic Landscaping of Primary Human Dental Stem Cells: Identification of SHED- and PDLSC-Specific Biomarkers

1
Center of Basic Research, Biomedical Research Foundation of the Academy of Athens, 11527 Athens, Greece
2
Proteomics Research Unit, Center of Basic Research II, Biomedical Research Foundation of the Academy of Athens, 11527 Athens, Greece
3
Section of Cell Biology and Biophysics, Department of Biology, National and Kapodistrian University of Athens, Panepistimiopolis, Zografou, 15701 Athens, Greece
4
Cellular and Molecular Biomechanics Unit, Department of Biological Chemistry, Medical School, National and Kapodistrian University of Athens, Medical School, 11527 Athens, Greece
5
Center for Engineering in Medicine, Massachusetts General Hospital, Department of Surgery, Harvard Medical School, Boston, MA 02114, USA
6
Shriners Burns Hospital, Boston, MA 02114, USA
7
Department of Endodontics, Dental School, National and Kapodistrian University of Athens, 11527 Athens, Greece
*
Author to whom correspondence should be addressed.
Received: 30 November 2017 / Revised: 25 December 2017 / Accepted: 29 December 2017 / Published: 5 January 2018
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Abstract

Dental stem cells (DSCs) have emerged as a promising tool for basic research and clinical practice. A variety of adult stem cell (ASC) populations can be isolated from different areas within the dental tissue, which, due to their cellular and molecular characteristics, could give rise to different outcomes when used in potential applications. In this study, we performed a high-throughput molecular comparison of two primary human adult dental stem cell (hADSC) sub-populations: Stem Cells from Human Exfoliated Deciduous Teeth (SHEDs) and Periodontal Ligament Stem Cells (PDLSCs). A detailed proteomic mapping of SHEDs and PDLSCs, via employment of nano-LC tandem-mass spectrometry (MS/MS) revealed 2032 identified proteins in SHEDs and 3235 in PDLSCs. In total, 1516 proteins were expressed in both populations, while 517 were unique for SHEDs and 1721 were exclusively expressed in PDLSCs. Further analysis of the recorded proteins suggested that SHEDs predominantly expressed molecules that are involved in organizing the cytoskeletal network, cellular migration and adhesion, whereas PDLSCs are highly energy-producing cells, vastly expressing proteins that are implicated in various aspects of cell metabolism and proliferation. Applying the Rho-GDI signaling pathway as a paradigm, we propose potential biomarkers for SHEDs and for PDLSCs, reflecting their unique features, properties and engaged molecular pathways. View Full-Text
Keywords: dental stem cells; PDLSC; SHED; proteomics; cellular functions; molecular pathways dental stem cells; PDLSC; SHED; proteomics; cellular functions; molecular pathways
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Taraslia, V.; Lymperi, S.; Pantazopoulou, V.; Anagnostopoulos, A.K.; Papassideri, I.S.; Basdra, E.K.; Bei, M.; Kontakiotis, E.G.; Tsangaris, G.T.; Stravopodis, D.J.; Anastasiadou, E. A High-Resolution Proteomic Landscaping of Primary Human Dental Stem Cells: Identification of SHED- and PDLSC-Specific Biomarkers. Int. J. Mol. Sci. 2018, 19, 158.

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