Next Article in Journal
The Potential Roles of the Apoptosis-Related Protein PDRG1 in Diapause Embryo Restarting of Artemia sinica
Next Article in Special Issue
Role of Microenvironment in Glioma Invasion: What We Learned from In Vitro Models
Previous Article in Journal
Metal Ion Effects on Aβ and Tau Aggregation
Previous Article in Special Issue
Molecular Determinants of Malignant Brain Cancers: From Intracellular Alterations to Invasion Mediated by Extracellular Vesicles
Article Menu
Issue 1 (January) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2018, 19(1), 127; https://doi.org/10.3390/ijms19010127

Differential Characterization of Temozolomide-Resistant Human Glioma Cells

1
Graduate Institute of Basic Medical Science, China Medical University, Taichung 40402, Taiwan
2
Graduate Institute of Clinical Medical Science, China Medical University, Taichung 40402, Taiwan
3
Neurosurgery Department, Taichung Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Taichung 42743, Taiwan
4
School of Medicine, Tzu Chi University, Hualien 97002, Taiwan
5
Department of Biotechnology, Asia University, Taichung 41354, Taiwan
6
Department of Pharmacology, School of Medicine, China Medical University, Taichung 40402, Taiwan
7
Graduate Institute of Biochemistry, National Chung Hsing University, Taichung 40249, Taiwan
8
Department of Photonics and Communication Engineering, Asia University, Taichung 41354, Taiwan
9
Department of Physiology, School of Medicine, China Medical University, Taichung 40402, Taiwan
*
Authors to whom correspondence should be addressed.
Received: 6 October 2017 / Revised: 29 December 2017 / Accepted: 29 December 2017 / Published: 2 January 2018
(This article belongs to the Special Issue Glioma Cell Invasion)
View Full-Text   |   Download PDF [3374 KB, uploaded 23 January 2018]   |  

Abstract

Glioblastoma multiforme (GBM) is the most common type of primary and malignant tumor occurring in the adult central nervous system. Temozolomide (TMZ) has been considered to be one of the most effective chemotherapeutic agents to prolong the survival of patients with glioblastoma. Many glioma cells develop drug-resistance against TMZ that is mediated by increasing O-6-methylguanine-DNA methyltransferase (MGMT) levels. The expression of connexin 43 was increased in the resistant U251 subline compared with the parental U251 cells. The expression of epithelial–mesenchymal transition (EMT)-associated regulators, including vimentin, N-cadherin, and β-catenin, was reduced in the resistant U251 subline. In addition, the resistant U251 subline exhibited decreased cell migratory activity and monocyte adhesion ability compared to the parental U251 cells. Furthermore, the resistant U251 subline also expressed lower levels of vascular cell adhesion molecule (VCAM)-1 after treatment with recombinant tumor necrosis factor (TNF)-α. These findings suggest differential characteristics in the drug-resistant GBM from the parental glioma cells. View Full-Text
Keywords: glioblastoma; temozolomide; connexin 43; drug-resistant glioblastoma; temozolomide; connexin 43; drug-resistant
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Lai, S.-W.; Huang, B.-R.; Liu, Y.-S.; Lin, H.-Y.; Chen, C.-C.; Tsai, C.-F.; Lu, D.-Y.; Lin, C. Differential Characterization of Temozolomide-Resistant Human Glioma Cells. Int. J. Mol. Sci. 2018, 19, 127.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top