Elongation of Axon Extension for Human iPSC-Derived Retinal Ganglion Cells by a Nano-Imprinted Scaffold
AbstractOptic neuropathies, such as glaucoma and Leber’s hereditary optic neuropathy (LHON) lead to retinal ganglion cell (RGC) loss and therefore motivate the application of transplantation technique into disease therapy. However, it is a challenge to direct the transplanted optic nerve axons to the correct location of the retina. The use of appropriate scaffold can promote the proper axon growth. Recently, biocompatible materials have been integrated into the medical field, such as tissue engineering and reconstruction of damaged tissues or organs. We, herein, utilized nano-imprinting to create a scaffold mimicking the in vitro tissue microarchitecture, and guiding the axonal growth and orientation of the RGCs. We observed that the robust, long, and organized axons of human induced pluripotent stem cell (iPSC)-derived RGCs projected axially along the scaffold grooves. The RGCs grown on the scaffold expressed the specific neuronal biomarkers indicating their proper functionality. Thus, based on our in vitro culture system, this device can be useful for the neurophysiological analysis and transplantation for ophthalmic neuropathy treatment. View Full-Text
Scifeed alert for new publicationsNever miss any articles matching your research from any publisher
- Get alerts for new papers matching your research
- Find out the new papers from selected authors
- Updated daily for 49'000+ journals and 6000+ publishers
- Define your Scifeed now
Yang, T.-C.; Chuang, J.-H.; Buddhakosai, W.; Wu, W.-J.; Lee, C.-J.; Chen, W.-S.; Yang, Y.-P.; Li, M.-C.; Peng, C.-H.; Chen, S.-J. Elongation of Axon Extension for Human iPSC-Derived Retinal Ganglion Cells by a Nano-Imprinted Scaffold. Int. J. Mol. Sci. 2017, 18, 2013.
Yang T-C, Chuang J-H, Buddhakosai W, Wu W-J, Lee C-J, Chen W-S, Yang Y-P, Li M-C, Peng C-H, Chen S-J. Elongation of Axon Extension for Human iPSC-Derived Retinal Ganglion Cells by a Nano-Imprinted Scaffold. International Journal of Molecular Sciences. 2017; 18(9):2013.Chicago/Turabian Style
Yang, Tien-Chun; Chuang, Jen-Hua; Buddhakosai, Waradee; Wu, Wen-Ju; Lee, Chen-Ju; Chen, Wun-Syuan; Yang, Yi-Ping; Li, Ming-Chia; Peng, Chi-Hsien; Chen, Shih-Jen. 2017. "Elongation of Axon Extension for Human iPSC-Derived Retinal Ganglion Cells by a Nano-Imprinted Scaffold." Int. J. Mol. Sci. 18, no. 9: 2013.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.