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Int. J. Mol. Sci. 2017, 18(9), 2013; doi:10.3390/ijms18092013

Elongation of Axon Extension for Human iPSC-Derived Retinal Ganglion Cells by a Nano-Imprinted Scaffold

1
Department of Medical Research, Taipei Veterans General Hospital, Taipei 11217, Taiwan
2
Institute of Pharmacology, National Yang-Ming University, Taipei 11221, Taiwan
3
Department of Biological Science and Technology, National Chiao Tung University, Hsinchu 30010, Taiwan
4
Department of Ophthalmology, Shin Kong Wu Ho-Su Memorial Hospital and Fu-Jen Catholic University, Taipei 11101, Taiwan
5
School of Medicine, National Yang-Ming University, Taipei 11221, Taiwan
6
Department of Ophthalmology, Taipei Veterans General Hospital, Taipei 11217, Taiwan
These authors contributed equally to the work.
*
Author to whom correspondence should be addressed.
Received: 15 August 2017 / Revised: 8 September 2017 / Accepted: 15 September 2017 / Published: 20 September 2017
(This article belongs to the Special Issue Disease Modeling Using Human Induced Pluripotent Stem Cells)
View Full-Text   |   Download PDF [7171 KB, uploaded 20 September 2017]   |  

Abstract

Optic neuropathies, such as glaucoma and Leber’s hereditary optic neuropathy (LHON) lead to retinal ganglion cell (RGC) loss and therefore motivate the application of transplantation technique into disease therapy. However, it is a challenge to direct the transplanted optic nerve axons to the correct location of the retina. The use of appropriate scaffold can promote the proper axon growth. Recently, biocompatible materials have been integrated into the medical field, such as tissue engineering and reconstruction of damaged tissues or organs. We, herein, utilized nano-imprinting to create a scaffold mimicking the in vitro tissue microarchitecture, and guiding the axonal growth and orientation of the RGCs. We observed that the robust, long, and organized axons of human induced pluripotent stem cell (iPSC)-derived RGCs projected axially along the scaffold grooves. The RGCs grown on the scaffold expressed the specific neuronal biomarkers indicating their proper functionality. Thus, based on our in vitro culture system, this device can be useful for the neurophysiological analysis and transplantation for ophthalmic neuropathy treatment. View Full-Text
Keywords: nano-imprinted; scaffold; RGC; axon outgrowth; elongation; orientation nano-imprinted; scaffold; RGC; axon outgrowth; elongation; orientation
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MDPI and ACS Style

Yang, T.-C.; Chuang, J.-H.; Buddhakosai, W.; Wu, W.-J.; Lee, C.-J.; Chen, W.-S.; Yang, Y.-P.; Li, M.-C.; Peng, C.-H.; Chen, S.-J. Elongation of Axon Extension for Human iPSC-Derived Retinal Ganglion Cells by a Nano-Imprinted Scaffold. Int. J. Mol. Sci. 2017, 18, 2013.

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