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Int. J. Mol. Sci. 2017, 18(9), 1956; doi:10.3390/ijms18091956

Alignment of Mitotic Chromosomes in Human Cells Involves SR-Like Splicing Factors Btf and TRAP150

1
Biomedical Sciences Ph.D. Program, Wright State University, Dayton, OH 45435, USA
2
Masters of Biological Sciences Program, Wright State University, Dayton, OH 45435, USA
3
Center for Genomics Research, Wright State University, Dayton, OH 45435, USA
4
Department of Biological Sciences, Wright State University, Dayton, OH 45435, USA
*
Authors to whom correspondence should be addressed.
Received: 1 September 2017 / Revised: 7 September 2017 / Accepted: 9 September 2017 / Published: 12 September 2017
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Serine-arginine-rich (SR) or SR-like splicing factors interact with exon junction complex proteins during pre-mRNA processing to promote mRNA packaging into mature messenger ribonucleoproteins (mRNPs) and to dictate mRNA stability, nuclear export, and translation. The SR protein family is complex, and while many classical SR proteins have well-defined mRNA processing functions, those of other SR-like proteins is unclear. Here, we show that depletion of the homologous non-classical serine-arginine-rich (SR) splicing factors Bcl2-associated transcription factor (Btf or BCLAF) and thyroid hormone receptor-associated protein of 150 kDa (TRAP150) causes mitotic defects. We hypothesized that the depletion of these SR-like factors affects mitosis indirectly through an altered expression of mitotic checkpoint regulator transcripts. We observed an altered abundance of transcripts that encode mitotic regulators and mitotic chromosome misalignment defects following Btf and/or TRAP150 depletion. We propose that, in addition to their previously reported roles in maintaining mRNA distribution, Btf and TRAP150 control the abundance of transcripts encoding mitotic regulators, thereby affecting mitotic progression in human cells. View Full-Text
Keywords: serine-arginine-rich (SR) proteins; Btf; TRAP150; pre-mRNA splicing; mitosis; cell cycle serine-arginine-rich (SR) proteins; Btf; TRAP150; pre-mRNA splicing; mitosis; cell cycle
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Varia, S.; Cheedu, D.; Markey, M.; Torres-Shafer, K.; Battini, V.P.; Bubulya, A.; Bubulya, P.A. Alignment of Mitotic Chromosomes in Human Cells Involves SR-Like Splicing Factors Btf and TRAP150. Int. J. Mol. Sci. 2017, 18, 1956.

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