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Int. J. Mol. Sci. 2017, 18(9), 1944; doi:10.3390/ijms18091944

Recent Advances in Experimental Whole Genome Haplotyping Methods

State Key Lab of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China
These authors contributed equally to this work.
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Received: 3 August 2017 / Revised: 1 September 2017 / Accepted: 5 September 2017 / Published: 11 September 2017
(This article belongs to the Special Issue Single Cell Technology)
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Abstract

Haplotype plays a vital role in diverse fields; however, the sequencing technologies cannot resolve haplotype directly. Pioneers demonstrated several approaches to resolve haplotype in the early years, which was extensively reviewed. Since then, numerous methods have been developed recently that have significantly improved phasing performance. Here, we review experimental methods that have emerged mainly over the past five years, and categorize them into five classes according to their maximum scale of contiguity: (i) encapsulation, (ii) 3D structure capture and construction, (iii) compartmentalization, (iv) fluorography, (v) long-read sequencing. Several subsections of certain methods are attached to each class as instances. We also discuss the relative advantages and disadvantages of different classes and make comparisons among representative methods of each class. View Full-Text
Keywords: haplotype; haplotyping; phasing; next generation sequencing haplotype; haplotyping; phasing; next generation sequencing
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Huang, M.; Tu, J.; Lu, Z. Recent Advances in Experimental Whole Genome Haplotyping Methods. Int. J. Mol. Sci. 2017, 18, 1944.

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