Next Article in Journal
Contribution of Intrinsic Lactate to Maintenance of Seizure Activity in Neocortical Slices from Patients with Temporal Lobe Epilepsy and in Rat Entorhinal Cortex
Next Article in Special Issue
The Contribution of Adipose Tissue-Derived Mesenchymal Stem Cells and Platelet-Rich Plasma to the Treatment of Chronic Equine Laminitis: A Proof of Concept
Previous Article in Journal
Genome-Wide Identification and Characterization of the GmSnRK2 Family in Soybean
Previous Article in Special Issue
Acceleration Mechanisms of Skin Wound Healing by Autologous Micrograft in Mice
Article Menu
Issue 9 (September) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2017, 18(9), 1829; doi:10.3390/ijms18091829

Fabrication of Innovative Silk/Alginate Microcarriers for Mesenchymal Stem Cell Delivery and Tissue Regeneration

1
Department of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100 Pavia, Italy
2
Department of Biomedical and Neuromotor Sciences (DIBINEM), University of Bologna, Via Zamboni 33, 40126 Bologna, Italy
3
Osteoarticular Regeneration Laboratory, 3rd Orthopaedic and Traumatologic Clinic, Rizzoli Orthopaedic Institute, Via Giulio Cesare Pupilli 1, 40136 Bologna, Italy
4
IRCCS Istituto Ortopedico Galeazzi, Via R. Galeazzi 4, 20161 Milan, Italy
5
Silk Division, Innovhub, Stazioni Sperimentali per l’Industria, Via G. Colombo 83, 20133 Milan, Italy
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 18 July 2017 / Revised: 9 August 2017 / Accepted: 15 August 2017 / Published: 23 August 2017
View Full-Text   |   Download PDF [8480 KB, uploaded 23 August 2017]   |  

Abstract

The aim of this study was to exploit silk fibroin’s properties to develop innovative composite microcarriers for mesenchymal stem cell (MSCs) adhesion and proliferation. Alginate microcarriers were prepared, added to silk fibroin solution, and then treated with ethanol to induce silk conformational transition. Microcarriers were characterized for size distribution, coating stability and homogeneity. Finally, in vitro cytocompatibility and suitability as delivery systems for MSCs were investigated. Results indicated that our manufacturing process is consistent and reproducible: silk/alginate microcarriers were stable, with spherical geometry, about 400 μm in average diameter, and fibroin homogeneously coated the surface. MSCs were able to adhere rapidly onto the microcarrier surface and to cover the surface of the microcarrier within three days of culture; moreover, on this innovative 3D culture system, stem cells preserved their metabolic activity and their multi-lineage differentiation potential. In conclusion, silk/alginate microcarriers represent a suitable support for MSCs culture and expansion. Since it is able to preserve MSCs multipotency, the developed 3D system can be intended for cell delivery, for advanced therapy and regenerative medicine applications. View Full-Text
Keywords: microcarriers; alginate; silk fibroin; mesenchymal stem cells; regenerative medicine; musculoskeletal tissues microcarriers; alginate; silk fibroin; mesenchymal stem cells; regenerative medicine; musculoskeletal tissues
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Perteghella, S.; Martella, E.; de Girolamo, L.; Perucca Orfei, C.; Pierini, M.; Fumagalli, V.; Pintacuda, D.V.; Chlapanidas, T.; Viganò, M.; Faragò, S.; Torre, M.L.; Lucarelli, E. Fabrication of Innovative Silk/Alginate Microcarriers for Mesenchymal Stem Cell Delivery and Tissue Regeneration. Int. J. Mol. Sci. 2017, 18, 1829.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top