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Int. J. Mol. Sci. 2017, 18(8), 1828; doi:10.3390/ijms18081828

Circadian and Light Regulated Expression of CBFs and their Upstream Signalling Genes in Barley

1
Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, 2462 Martonvásár, Hungary
2
Festetics Doctoral School, Georgikon Faculty, University of Pannonia, 8360 Keszthely, Hungary
3
Institute of Plant Biology, Biological Research Centre, Hungarian Academy of Sciences, 6726 Szeged, Hungary
4
Department of Genetics, Faculty of Sciences and Informatics, University of Szeged, 6726 Szeged, Hungary
*
Author to whom correspondence should be addressed.
Received: 5 July 2017 / Revised: 10 August 2017 / Accepted: 19 August 2017 / Published: 22 August 2017
(This article belongs to the Special Issue Abiotic Stress and Gene Networks in Plants 2017)
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Abstract

CBF (C-repeat binding factor) transcription factors show high expression levels in response to cold; moreover, they play a key regulatory role in cold acclimation processes. Recently, however, more and more information has led to the conclusion that, apart from cold, light—including its spectra—also has a crucial role in regulating CBF expression. Earlier, studies established that the expression patterns of some of these regulatory genes follow circadian rhythms. To understand more of this complex acclimation process, we studied the expression patterns of the signal transducing pathways, including signal perception, the circadian clock and phospholipid signalling pathways, upstream of the CBF gene regulatory hub. To exclude the confounding effect of cold, experiments were carried out at 22 °C. Our results show that the expression of genes implicated in the phospholipid signalling pathway follow a circadian rhythm. We demonstrated that, from among the tested CBF genes expressed in Hordeum vulgare (Hv) under our conditions, only the members of the HvCBF4-phylogenetic subgroup showed a circadian pattern. We found that the HvCBF4-subgroup genes were expressed late in the afternoon or early in the night. We also determined the expression changes under supplemental far-red illumination and established that the transcript accumulation had appeared four hours earlier and more intensely in several cases. Based on our results, we propose a model to illustrate the effect of the circadian clock and the quality of the light on the elements of signalling pathways upstream of the HvCBFs, thus integrating the complex regulation of the early cellular responses, which finally lead to an elevated abiotic stress tolerance. View Full-Text
Keywords: barley; circadian rhythm; gene expression; calcium signalling; HvCBF-phylogenetic subgroups; light quality; low red/far-red ratio barley; circadian rhythm; gene expression; calcium signalling; HvCBF-phylogenetic subgroups; light quality; low red/far-red ratio
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Gierczik, K.; Novák, A.; Ahres, M.; Székely, A.; Soltész, A.; Boldizsár, Á.; Gulyás, Z.; Kalapos, B.; Monostori, I.; Kozma-Bognár, L.; Galiba, G.; Vágújfalvi, A. Circadian and Light Regulated Expression of CBFs and their Upstream Signalling Genes in Barley. Int. J. Mol. Sci. 2017, 18, 1828.

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