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Int. J. Mol. Sci. 2017, 18(8), 1779; doi:10.3390/ijms18081779

Enhanced Cell Growth of Adipocyte-Derived Mesenchymal Stem Cells Using Chemically-Defined Serum-Free Media

1
Xcell Therapeutics, Hanhwa Biz Metro Building, 242 Digital-ro, Guro-gu, Seoul 152-733, Korea
2
College of Pharmacy, Seoul National University, Kwanak-ro, Kwanak-gu, Seoul 151-742, Korea
3
ABION Inc., Hanhwa Biz Metro Building, 242 Digital-ro, Guro-gu, Seoul 152-733, Korea
4
Research Institute of Pharmaceutical Sciences, Seoul National University, Kwanak-ro, Kwanak-gu, Seoul 151-742, Korea
5
Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science and Technology, Seoul National University, Seoul 08826, Korea
6
Department of Pharmacy, College of Pharmacy, Ajou University, Worldcup-ro, 206, Yeongtong-gu, Suwon 16499, Korea
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 5 August 2017 / Revised: 12 August 2017 / Accepted: 14 August 2017 / Published: 16 August 2017
(This article belongs to the Special Issue Stem Cell Research)
View Full-Text   |   Download PDF [3878 KB, uploaded 16 August 2017]   |  

Abstract

The multipotency and anti-inflammatory effects of mesenchymal stem cells (MSCs) make them attractive for cell therapy in regenerative medicine. A large number of MSCs is required for efficient therapy owing to the low homing efficiency of MSCs to target sites. Furthermore, owing to limitations in obtaining sufficient amounts of MSCs, in vitro expansion of MSCs that preserves their differentiation and proliferative potential is essential. The animal factor included in culture media also limits clinical application. In this study, adipose-derived MSCs showed a significantly higher proliferation rate in STK2, a chemically-defined medium, than in DMEM/FBS. The expression of MSC surface markers was increased in the culture using STK2 compared to that using DMEM/FBS. Tri-lineage differentiation analyses showed that MSCs cultured in STK2 were superior to those cultured in DMEM/FBS. In addition, MSCs cultured in STK2 showed a reduced senescence rate, small and homogenous cell size, and were more genetically stable compared to those cultured in DMEM/FBS. Furthermore, secretome analysis showed that the expression of factors related to proliferation/migration, anti-inflammation, and differentiation were increased in STK2 culture medium compared to DMEM/FBS. Taken together, these results suggest that culture using STK2 medium offers many advantages through which it is possible to obtain safer, superior, and larger numbers of MSCs. View Full-Text
Keywords: mesenchymal stem cell; multipotency; chemically-defined medium; proliferation; differentiation; secretome mesenchymal stem cell; multipotency; chemically-defined medium; proliferation; differentiation; secretome
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Lee, M.-S.; Youn, C.; Kim, J.H.; Park, B.J.; Ahn, J.; Hong, S.; Kim, Y.-D.; Shin, Y.K.; Park, S.G. Enhanced Cell Growth of Adipocyte-Derived Mesenchymal Stem Cells Using Chemically-Defined Serum-Free Media. Int. J. Mol. Sci. 2017, 18, 1779.

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