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Int. J. Mol. Sci. 2017, 18(8), 1722; doi:10.3390/ijms18081722

Platelet-Rich Fibrin Facilitates Rabbit Meniscal Repair by Promoting Meniscocytes Proliferation, Migration, and Extracellular Matrix Synthesis

1
Graduate Institute of Clinical Medicine, College of Medicine, National Taiwan University, Taipei 10002, Taiwan
2
Department of Orthopedics, Shuang Ho Hospital, Taipei Medical University, New Taipei City 23561, Taiwan
3
Department of Orthopedics, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan
4
Department of Post-Baccalaureate Veterinary Medicine, Asia University, 500, Lioufeng Road, Wufeng, Taichung 41354, Taiwan
5
Research Center for Developmental Biology and Regenerative Medicine, National Taiwan University, Taipei 10617, Taiwan
6
Department of Otolaryngology, National Taiwan University Hospital and College of Medicine, Taipei 10051, Taiwan
7
Department of Radiology, Wan-Fang Hospital, Taipei Medical University, 111 Hsing Long Road, Section 3, Taipei 11651, Taiwan
8
Department of Medical Imaging and Radiological Technology, Yuanpei University, Hsinchu 30015, Taiwan
9
Department of Orthopedics, National Taiwan University Hospital, Taipei 10048, Taiwan
10
Department of Pathology, Taipei Medical University Hospital, Taipei Medical University, Taipei 11031, Taiwan
11
Department of Radiology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan
*
Authors to whom correspondence should be addressed.
Received: 5 July 2017 / Revised: 28 July 2017 / Accepted: 3 August 2017 / Published: 7 August 2017
(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)
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Abstract

Although platelet-rich fibrin (PRF) has been used in clinical practice for some time, to date, few studies reveal its role as a bioactive scaffold in facilitating meniscal repair. Here, the positive anabolic effects of PRF on meniscocytes harvested from the primary culture of a rabbit meniscus were revealed. The rabbit meniscocytes were cultured with different concentrations of PRF-conditioned medium, and were evaluated for their ability to stimulate cell migration, proliferation, and extracellular matrix formation. In vivo, meniscal defects were created via an established rabbit animal model and were evaluated by a histology-based four-stage scoring system to validate the treatment outcome three months postoperatively. The in vitro results showed that PRF could induce cellular migration and promote proliferation and meniscocyte extracellular matrix (ECM) synthesis of cultured meniscocytes. In addition, PRF increased the formation and deposition of cartilaginous matrix produced by cultured meniscocytes. Morphological and histological evaluations demonstrated that PRF could facilitate rabbit meniscal repair. The data highlight the potential utility of using PRF in augmenting the healing of meniscal injuries. These advantages would benefit clinical translation, and are a potential new treatment strategy for meniscal repair. View Full-Text
Keywords: meniscocytes; meniscus; platelet-rich fibrin; regeneration meniscocytes; meniscus; platelet-rich fibrin; regeneration
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MDPI and ACS Style

Wong, C.-C.; Kuo, T.-F.; Yang, T.-L.; Tsuang, Y.-H.; Lin, M.-F.; Chang, C.-H.; Lin, Y.-H.; Chan, W.P. Platelet-Rich Fibrin Facilitates Rabbit Meniscal Repair by Promoting Meniscocytes Proliferation, Migration, and Extracellular Matrix Synthesis. Int. J. Mol. Sci. 2017, 18, 1722.

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