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Int. J. Mol. Sci. 2017, 18(8), 1661; doi:10.3390/ijms18081661

Functional and Promoter Analysis of ChiIV3, a Chitinase of Pepper Plant, in Response to Phytophthora capsici Infection

1,2,†
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1,2,†
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and
1,2,*
1
Ministry of Education Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou 350002, China
2
College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 19 June 2017 / Revised: 28 July 2017 / Accepted: 30 July 2017 / Published: 1 August 2017
(This article belongs to the Section Molecular Botany)
View Full-Text   |   Download PDF [2863 KB, uploaded 1 August 2017]   |  

Abstract

Despite the involvement of many members of the chitinase family in plant immunity, the precise functions of the majority of the members remain poorly understood. Herein, the gene ChiIV3 in Capsicum annuum encoding a chitinase protein containing a chitin binding domain and targeting to the plasma membrane was found to be induced by Phytophthora capsici inoculation (PCI) and applied chitin treatment. Besides its direct inhibitory effect on growth of Phytophthora capsici (P. capsici), ChiIV3 was also found by virus-induced gene silencing (VIGS) and transient overexpression (TOE) in pepper plants to act as a positive regulator of plant cell death and in triggering defense signaling and upregulation of PR (pathogenesis related) genes against PCI. A 5′ deletion assay revealed that pChiIV3−712 to −459 bp was found to be sufficient for ChiIV3’ response to PCI. Furthermore, a mutation assay indicated that W-box−466 to −461 bp in pChiIV3−712 to −459 bp was noted to be the PCI-responsible element. These results collectively suggest that ChiIV3 acts as a likely antifungal protein and as a receptor for unidentified chitin in planta to trigger cell death and defense signaling against PCI. View Full-Text
Keywords: Capsicum annuum; chitinase; Phytophthora capsici; cell death; promoter Capsicum annuum; chitinase; Phytophthora capsici; cell death; promoter
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Liu, Z.; Shi, L.; Yang, S.; Lin, Y.; Weng, Y.; Li, X.; Hussain, A.; Noman, A.; He, S. Functional and Promoter Analysis of ChiIV3, a Chitinase of Pepper Plant, in Response to Phytophthora capsici Infection. Int. J. Mol. Sci. 2017, 18, 1661.

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