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Int. J. Mol. Sci. 2017, 18(8), 1611; doi:10.3390/ijms18081611

Expression of Serum Exosomal and Esophageal MicroRNA in Rat Reflux Esophagitis

1
Department of Gastroenterology, Osaka City University Graduate School of Medicine, Osaka 545-8585, Japan
2
Department of Hepatology, Osaka City University Graduate School of Medicine, Osaka 545-8585, Japan
3
Department of Physics, Chuo University, Tokyo 112-8551, Japan
*
Author to whom correspondence should be addressed.
Received: 9 June 2017 / Revised: 18 July 2017 / Accepted: 20 July 2017 / Published: 25 July 2017
(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)
View Full-Text   |   Download PDF [1662 KB, uploaded 25 July 2017]   |  

Abstract

Gastroesophageal reflux disease (GERD) is a common upper gastrointestinal disease. However, the role of exosomal microRNAs (miRNAs) and esophageal miRNAs in GERD has not been studied. A rat model of acid reflux esophagitis was used to establish a novel diagnosis marker for GERD and examine dynamics of miRNA expression in GERD. Rats were sacrificed 3 (acute phase), 7 (sub-acute phase) and 21 days (chronic phase) after induction of esophagitis. Exosomes were extracted from serum, and the expression patterns of serum miRNAs were analyzed. Four upregulated miRNAs (miR-29a-3p, 128-3p, 223-3p and 3473) were identified by microarray analysis. The expression levels of exosomal miR-29a-3p were significantly higher in the chronic phase of reflux esophagitis compared with controls, and increased expression of miR-29a-3p was specific to chronic reflux esophagitis. Esophageal miR-223-3p expression was higher compared with controls, and gradually decreased from acute to chronic phase in esophagitis. In conclusion, exosomal miR-29a-3p and esophageal miR-223-3p might play roles in GERD. View Full-Text
Keywords: gastroesophageal reflux disease; non-erosive reflux disease; reflux esophagitis; microRNA; exosome gastroesophageal reflux disease; non-erosive reflux disease; reflux esophagitis; microRNA; exosome
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Uemura, R.; Murakami, Y.; Hashimoto, A.; Sawada, A.; Otani, K.; Taira, K.; Hosomi, S.; Nagami, Y.; Tanaka, F.; Kamata, N.; Yamagami, H.; Tanigawa, T.; Watanabe, T.; Taguchi, Y.-H.; Fujiwara, Y. Expression of Serum Exosomal and Esophageal MicroRNA in Rat Reflux Esophagitis. Int. J. Mol. Sci. 2017, 18, 1611.

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