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Int. J. Mol. Sci. 2017, 18(7), 1573; doi:10.3390/ijms18071573

Oleic Acid Uptake Reveals the Rescued Enterocyte Phenotype of Colon Cancer Caco-2 by HT29-MTX Cells in Co-Culture Mode

1
Univ-Lyon, CarMeN Laboratory, Inserm U1060, INRA U1397, Université Claud Bernard Lyon1, INSA Lyon, Fr-69100 Villeurbanne, France
2
Institut CBMN (Chimie et Biologie des Membranes & Nanoobjets)-UMR 5248 (CNRS-Bordeaux INP-Université de Bordeaux)-Equipe ClipIN-Bât B14-Ave G. St Hilaire, 33600 Pessac, France
*
Author to whom correspondence should be addressed.
Received: 30 May 2017 / Revised: 7 July 2017 / Accepted: 16 July 2017 / Published: 20 July 2017
(This article belongs to the Special Issue Alterations to Signalling Pathways in Cancer Cells)
View Full-Text   |   Download PDF [5528 KB, uploaded 20 July 2017]   |  

Abstract

Gastrointestinal epithelium is the unique route for nutrients and for many pharmaceuticals to enter the body. The present study aimed to analyze precisely whether co-culture of two colon cancer cell lines, mucus-producing cells HT29-MTX and enterocyte-like Caco-2 cells, ameliorate differentiation into an in vitro intestinal barrier model and the signaling pathways involved. Differentiated Caco-2 cells gene datasets were compared first to intestinal or cancer phenotypes and second to signaling pathway gene datasets. Experimental validations were performed in real-time experiments, immunochemistry, and gene expression analyses on Caco-2 versus co-cultures of Caco-2 and HT29-MTX (10%) cells. Partial maintenance of cancer-cell phenotype in differentiated Caco-2 cells was confirmed and fatty acids merged as potential regulators of cancer signaling pathways. HT29-MTX cells induced morphological changes in Caco-2 cells, slightly increased their proliferation rate and profoundly modified gene transcription of phenotype markers, fatty acid receptors, intracellular transporters, and lipid droplet components as well as functional responses to oleic acid. In vitro, enterocyte phenotype was rescued partially by co-culture of cancer cells with goblet cells and completed through oleic acid interaction with signaling pathways dysregulated in cancer cells. View Full-Text
Keywords: cancer cell lines; co-culture; fatty acids; gene datasets; signaling pathways; intestinal barrier cancer cell lines; co-culture; fatty acids; gene datasets; signaling pathways; intestinal barrier
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Berger, E.; Nassra, M.; Atgié, C.; Plaisancié, P.; Géloën, A. Oleic Acid Uptake Reveals the Rescued Enterocyte Phenotype of Colon Cancer Caco-2 by HT29-MTX Cells in Co-Culture Mode. Int. J. Mol. Sci. 2017, 18, 1573.

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