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A Panel of MicroRNAs as Diagnostic Biomarkers for the Identification of Prostate Cancer
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Int. J. Mol. Sci. 2017, 18(7), 1512; doi:10.3390/ijms18071512

The Prostate Cancer Cells Resistant to Docetaxel as in vitro Model for Discovering MicroRNAs Predictive of the Onset of Docetaxel Resistance

1
Non-Coding RNA Laboratory, Institute of Clinical Physiology (IFC), National Research Council (CNR), via G. Moruzzi 1, 56124 Pisa, Italy
2
Laboratory for Integrative System Medicine (LISM), Institute of Informatics and Telematics (IIT), National Research Council (CNR), via G. Moruzzi 1, 56124 Pisa, Italy
3
Department of Oncology, San Donato Hospital, Azienda USL Toscana Sud-Est, via P. Nenni 20, 52100 Arezzo, Italy
4
Istituto Toscano Tumori (ITT), via T. Alderotti 26/N, 50139 Firenze, Italy
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 19 June 2017 / Revised: 5 July 2017 / Accepted: 5 July 2017 / Published: 13 July 2017
(This article belongs to the Special Issue Diagnostic, Prognostic and Predictive Biomarkers in Prostate Cancer)
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Abstract

On the grounds that miRNAs present in the blood of prostate cancer (PCa) patients are released in the growth medium by PCa cells, it is conceivable that PCa cells resistant to docetaxel (DCT) (DCTR) will release miRNAs that may be found in PCa patients under DCT therapy if resistant PCa cells appear. We isolated DCTR clones respectively from 22Rv1 and DU-145 PCa cell lines and performed through next-generation sequencing (NGS) the miRNAs profiles of the released miRNAs. The analysis of the NGS data identified 105 and 1 miRNAs which were differentially released in the growth medium of the 22Rv1/DCTR and DU-145/DCTR clones, respectively. Using additional filters, we selected 12 and 1 miRNA more released by all 22Rv1/DCTR and DU-145/DCTR clones, respectively. Moreover, we showed that 6 of them were more represented in the growth medium of the DCTR cells than the ones of DCT-treated cells. We speculated that they have the pre-requisite to be tested as predictive biomarkers of the DCT resistance in PCa patients under DCT therapy. We propose the utilization of clones resistant to a given drug as in vitro model to identify the differentially released miRNAs, which in perspective could be tested as predictive biomarkers of drug resistance in tumor patients under therapy. View Full-Text
Keywords: prostate cancer cell lines; docetaxel resistance; circulating miRNAs; predictive biomarkers prostate cancer cell lines; docetaxel resistance; circulating miRNAs; predictive biomarkers
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Bascetta, L.; Oliviero, A.; D’Aurizio, R.; Evangelista, M.; Mercatanti, A.; Pellegrini, M.; Marrocolo, F.; Bracarda, S.; Rizzo, M. The Prostate Cancer Cells Resistant to Docetaxel as in vitro Model for Discovering MicroRNAs Predictive of the Onset of Docetaxel Resistance. Int. J. Mol. Sci. 2017, 18, 1512.

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