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Int. J. Mol. Sci. 2017, 18(7), 1433; doi:10.3390/ijms18071433

Multi-Approach Analysis for the Identification of Proteases within Birch Pollen

1
Department of Molecular Biology, University of Salzburg, Salzburg 5020, Austria
2
Department of Breeding Informatics, Georg August-Universität Göttingen, Göttingen 37073, Germany
*
Author to whom correspondence should be addressed.
Received: 26 May 2017 / Revised: 28 June 2017 / Accepted: 30 June 2017 / Published: 4 July 2017
(This article belongs to the Special Issue Proteolysis in Allergic Sensitization and Th2 Response)
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Abstract

Birch pollen allergy is highly prevalent, with up to 100 million reported cases worldwide. Proteases in such allergen sources have been suggested to contribute to primary sensitisation and exacerbation of allergic disorders. Until now the protease content of Betula verrucosa, a birch species endemic to the northern hemisphere has not been studied in detail. Hence, we aim to identify and characterise pollen and bacteria-derived proteases found within birch pollen. The pollen transcriptome was constructed via de novo transcriptome sequencing and analysis of the proteome was achieved via mass spectrometry; a cross-comparison of the two databases was then performed. A total of 42 individual proteases were identified at the proteomic level. Further clustering of proteases into their distinct catalytic classes revealed serine, cysteine, aspartic, threonine, and metallo-proteases. Further to this, protease activity of the pollen was quantified using a fluorescently-labelled casein substrate protease assay, as 0.61 ng/mg of pollen. A large number of bacterial strains were isolated from freshly collected birch pollen and zymographic gels with gelatinase and casein, enabled visualisation of proteolytic activity of the pollen and the collected bacterial strains. We report the successful discovery of pollen and bacteria-derived proteases of Betula verrucosa. View Full-Text
Keywords: birch pollen; allergy; protease; proteome; transcriptome; zymogram birch pollen; allergy; protease; proteome; transcriptome; zymogram
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McKenna, O.E.; Posselt, G.; Briza, P.; Lackner, P.; Schmitt, A.O.; Gadermaier, G.; Wessler, S.; Ferreira, F. Multi-Approach Analysis for the Identification of Proteases within Birch Pollen. Int. J. Mol. Sci. 2017, 18, 1433.

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