Next Article in Journal
Propolis Diterpenes as a Remarkable Bio-Source for Drug Discovery Development: A Review
Previous Article in Journal
Interactions between a Heparin Trisaccharide Library and FGF-1 Analyzed by NMR Methods
Article Menu
Issue 6 (June) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2017, 18(6), 1291; doi:10.3390/ijms18061291

Optimization of Polyplex Formation between DNA Oligonucleotide and Poly(ʟ-Lysine): Experimental Study and Modeling Approach

1
Center of Advanced Research in Bionanocojugates and biopolymers, “Petru Poni” Institute of Macromolecular Chemistry, Iasi, Romania Aleea Grigore Ghica Voda 41A, 70487 Iasi, Romania
2
Department of Inorganic Polymers, “Petru Poni” Institute of Macromolecular Chemistry, Iasi, Romania Aleea Grigore Ghica Voda 41A, 70487 Iasi, Romania
*
Author to whom correspondence should be addressed.
Received: 11 May 2017 / Revised: 7 June 2017 / Accepted: 13 June 2017 / Published: 17 June 2017
(This article belongs to the Section Biochemistry and Molecular Biology)
View Full-Text   |   Download PDF [4531 KB, uploaded 19 June 2017]   |  

Abstract

The polyplexes formed by nucleic acids and polycations have received a great attention owing to their potential application in gene therapy. In our study, we report experimental results and modeling outcomes regarding the optimization of polyplex formation between the double-stranded DNA (dsDNA) and poly(ʟ-Lysine) (PLL). The quantification of the binding efficiency during polyplex formation was performed by processing of the images captured from the gel electrophoresis assays. The design of experiments (DoE) and response surface methodology (RSM) were employed to investigate the coupling effect of key factors (pH and N/P ratio) affecting the binding efficiency. According to the experimental observations and response surface analysis, the N/P ratio showed a major influence on binding efficiency compared to pH. Model-based optimization calculations along with the experimental confirmation runs unveiled the maximal binding efficiency (99.4%) achieved at pH 5.4 and N/P ratio 125. To support the experimental data and reveal insights of molecular mechanism responsible for the polyplex formation between dsDNA and PLL, molecular dynamics simulations were performed at pH 5.4 and 7.4. View Full-Text
Keywords: DNA; modeling; optimization; poly(ʟ-Lysine) DNA; modeling; optimization; poly(ʟ-Lysine)
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Vasiliu, T.; Cojocaru, C.; Rotaru, A.; Pricope, G.; Pinteala, M.; Clima, L. Optimization of Polyplex Formation between DNA Oligonucleotide and Poly(ʟ-Lysine): Experimental Study and Modeling Approach. Int. J. Mol. Sci. 2017, 18, 1291.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top