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Int. J. Mol. Sci. 2017, 18(6), 1270; doi:10.3390/ijms18061270

Terpenoids from Platostoma rotundifolium (Briq.) A. J. Paton Alter the Expression of Quorum Sensing-Related Virulence Factors and the Formation of Biofilm in Pseudomonas aeruginosa PAO1

1
Laboratory of Plant Biotechnology, Université Libre de Bruxelles, rue des Professeurs Jeener et Brachet 12, 6041 Gosselies, Belgium
2
Laboratoire de Pharmacognosie, Bromatologie et Nutrition Humaine, Faculté de Pharmacie, Université Libre de Bruxelles, CP 205/09, Boulevard du Triomphe, 1050 Bruxelles, Belgium
3
Centre de Recherche Universitaire en Pharmacopée et Médecine Traditionnelle (CRUPHAMET), Faculté des Sciences, Université du Burundi, BP 2700 Bujumbura, Burundi
4
Unit of Therapeutic Chemistry and Pharmacognosy, Université de Mons (UMONS), Bâtiment VI, Chemin du Champ de Mars 25, 7000 Mons, Belgium
5
Laboratoire de Biotechnologie et Microbiologie, Département de Biochimie Fondamentale et Appliquée, Faculté des Sciences, Université d’Antananarivo (UA), BP 906, Antananarivo 101, Madagascar
6
Département de Pharmacochimie Moléculaire, Université Grenoble Alpes, 38000 Grenoble, France
7
Département de Pharmacochimie Moléculaire, Centre National de Recherche Scientifique, 38000 Grenoble, France
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 7 May 2017 / Revised: 30 May 2017 / Accepted: 7 June 2017 / Published: 14 June 2017
(This article belongs to the Section Bioactives and Nutraceuticals)
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Abstract

Platostoma rotundifolium (Briq.) A. J. Paton aerial parts are widely used in Burundi traditional medicine to treat infectious diseases. In order to investigate their probable antibacterial activities, crude extracts from P. rotundifolium were assessed for their bactericidal and anti-virulence properties against an opportunistic bacterial model, Pseudomonas aeruginosa PAO1. Whereas none of the tested extracts exert bacteriostatic and/or bactericidal proprieties, the ethyl acetate and dichloromethane extracts exhibit anti-virulence properties against Pseudomonas aeruginosa PAO1 characterized by an alteration in quorum sensing gene expression and biofilm formation without affecting bacterial viability. Bioguided fractionation of the ethyl acetate extract led to the isolation of major anti-virulence compounds that were identified from nuclear magnetic resonance and high-resolution molecular spectroscopy spectra as cassipourol, β-sitosterol and α-amyrin. Globally, cassipourol and β-sitosterol inhibit quorum sensing-regulated and -regulatory genes expression in las and rhl systems without affecting the global regulators gacA and vfr, whereas α-amyrin had no effect on the expression of these genes. These terpenoids disrupt the formation of biofilms at concentrations down to 12.5, 50 and 50 µM for cassipourol, β-sitosterol and α-amyrin, respectively. Moreover, these terpenoids reduce the production of total exopolysaccharides and promote flagella-dependent motilities (swimming and swarming). The isolated terpenoids exert a wide range of inhibition processes, suggesting a complex mechanism of action targeting P. aeruginosa virulence mechanisms which support the wide anti-infectious use of this plant species in traditional Burundian medicine. View Full-Text
Keywords: anti-virulence; biofilm; Platostoma rotundifolium; Lamiaceae; Pseudomonas aeruginosa; quorum sensing anti-virulence; biofilm; Platostoma rotundifolium; Lamiaceae; Pseudomonas aeruginosa; quorum sensing
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Rasamiravaka, T.; Ngezahayo, J.; Pottier, L.; Ribeiro, S.O.; Souard, F.; Hari, L.; Stévigny, C.; Jaziri, M.E.; Duez, P. Terpenoids from Platostoma rotundifolium (Briq.) A. J. Paton Alter the Expression of Quorum Sensing-Related Virulence Factors and the Formation of Biofilm in Pseudomonas aeruginosa PAO1. Int. J. Mol. Sci. 2017, 18, 1270.

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