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Int. J. Mol. Sci. 2017, 18(6), 1227; doi:10.3390/ijms18061227

Identification of Novel Placentally Expressed Aspartic Proteinase in Humans

1
Department of Human Physiology, Faculty of Medical Sciences, University of Warmia and Mazury in Olsztyn, Warszawska Str 30, 10-082 Olsztyn, Poland
2
Department of Gynecology and Obstetrics, Faculty of Medical Sciences, University of Warmia and Mazury in Olsztyn, Niepodleglosci Str 44, 10-045 Olsztyn, Poland
3
Department of Animal Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego Str 1A, 10-719 Olsztyn, Poland
*
Author to whom correspondence should be addressed.
Academic Editor: Cheorl-Ho Kim
Received: 19 March 2017 / Revised: 2 June 2017 / Accepted: 5 June 2017 / Published: 8 June 2017
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

This study presents pioneering data concerning the human pregnancy-associated glycoprotein-Like family, identified in the genome, of the term placental transcriptome and proteome. RNA-seq allowed the identification of 1364 bp hPAG-L/pep cDNA with at least 56.5% homology with other aspartic proteinases (APs). In silico analyses revealed 388 amino acids (aa) of full-length hPAG-L polypeptide precursor, with 15 aa-signal peptide, 47 aa-blocking peptide and 326 aa-mature protein, and two Asp residues (D), specific for a catalytic cleft of the APs (VVFDTGSSNLWV91-102 and AIVDTGTSLLTG274-285). Capillary sequencing identified 9330 bp of the hPAG-L gene (Gen Bank Acc. No. KX533473), composed of nine exons and eight introns. Heterologous Western blotting revealed the presence of one dominant 60 kDa isoform of the hPAG-L amongst cellular placental proteins. Detection with anti-pPAG-P and anti-Rec pPAG2 polyclonals allowed identification of the hPAG-L proteins located within regions of chorionic villi, especially within the syncytiotrophoblast of term singleton placentas. Our novel data extend the present knowledge about the human genome, as well as placental transcriptome and proteome during term pregnancy. Presumably, this may contribute to establishing a new diagnostic tool for examination of some disturbances during human pregnancy, as well as growing interest from both scientific and clinical perspectives. View Full-Text
Keywords: cDNA; gDNA; human PAG-L; placenta; trophectoderm cDNA; gDNA; human PAG-L; placenta; trophectoderm
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Majewska, M.; Lipka, A.; Panasiewicz, G.; Gowkielewicz, M.; Jozwik, M.; Majewski, M.K.; Szafranska, B. Identification of Novel Placentally Expressed Aspartic Proteinase in Humans. Int. J. Mol. Sci. 2017, 18, 1227.

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